Patterned and switchable surfaces for biomaterial applications

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Chapter 4 – Formation of a chemically patterned substrate for cell microarray applications(A)(B)(C)(D)Figure 4.4. Fluorescence images of polymer arrays before (A and B) and after (C andD) overnight washing. For (A) and (C), from left to right each array setcorresponds to PAA, PEI, PLL and PVP printed onto a PEG coating at aninitial polymer concentration of 4 mg/mL. Across each row from left to rightin each array set the concentration of NSANB incubated with the polymerbefore spotting is altered between 2.0, 1.0, 0.5 and 0.0 mg/mL. Each columnis a set of three replicates. For (B) and (D), each array set is of PAAfunctionalised with NSANB printed onto a PEG coating at an initial polymerconcentration of 4 mg/mL. Across each row from left to right in each arrayset the concentration of NSANB incubated with the polymer before spottingis altered between 2.0, 1.0, 0.5 and 0.0 mg/mL. Each column is a set of threereplicates. The centre-to-centre distance between spots is 750 m.4-146
Thickness (nm)Thickness (nm)Thickness (nm)Thickness (nm)Andrew Hook – Patterned and switchable surfaces for biomaterial applications(A)(C)15001200.5 1 1.5 2906030040200 0.5 1 1.5 2Polymer spottingconcentration (mg/ml)600 0.5 1 1.5 2(B)(D)70600 0.5 1 1.5 2504030201000 0.5 1 1.5 2Polymer spottingconcentration (mg/ml)2502000 0.5 1 1.5 21501005000 0.5 1 1.5 2Polymer spottingconcentration (mg/ml)00 0.5 1 1.5 2Polymer spottingconcentration (mg/ml)Figure 4.5. Height measurements of UV irradiated polymer spots determined fromprofilometry before (■) and after () washing of polymer spots of PAA (A),PEIc (B), PLLc (C) and PVP (D) printed from polymer solutions of variedconcentration, all containing 1 mg/ml NSANB solution.4-147
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Patterned andswitchable surfacesfor
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TABLE OF CONTENTSTABLE OF CONTENTS
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Andrew Hook - Patterned and switcha
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CHAPTER 1.INTRODUCTION1The content
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Chapter 1 - Introductionconsiderabl
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Chapter 1 - Introductioninteraction
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Chapter 1 - IntroductionIn general,
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Chapter 1 - IntroductionFor some ap
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Chapter 1 - Introductionangles rang
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Chapter 1 - Introductionof understa
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Chapter 1 - IntroductionSeveral str
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Chapter 1 - Introductioncues to con
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Chapter 1 - Introductionby controll
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Chapter 1 - Introduction1.2.2. Soft
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Chapter 1 - Introductionsubstrate.
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Chapter 1 - Introduction(A)(B)Figur
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Chapter 1 - Introduction(A)(B)(C)(C
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Chapter 1 - Introductioncomplex gen
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Chapter 1 - Introductionmorphology
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Chapter 1 - Introduction(A)(B)(C)(D
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Chapter 1 - Introductionthe LCST, a
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Chapter 1 - IntroductionHyun et al.
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Chapter 1 - Introductionmicelles. T
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Chapter 1 - Introduction1.4.1. DNA
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Chapter 1 - IntroductionA major pro
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Chapter 1 - Introductionattached to
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Chapter 1 - IntroductionTable 1.1.
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Chapter 1 - Introductionefficiencie
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Chapter 1 - Introductionachieved by
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Chapter 1 - IntroductionTable 1.2.
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Chapter 1 - IntroductionOnce releas
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Chapter 1 - Introductionimaging not
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Chapter 1 - IntroductionA number of
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CHAPTER 2.SPATIALLY CONTROLLED ELEC
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CHAPTER 6.OVERALL CONCLUSIONS6.6An
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Chapter 6 - Overall conclusionsThes
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Chapter 6 - Overall conclusionsnew
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Force (nN)Force (nN)Force (nN)Force
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Force (nN)Force (nN)Andrew Hook - P
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Patterned and switchable surfaces for biomaterial applications

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