Patterned and switchable surfaces for biomaterial applications

Chapter 4 – Formation of a chemically patterned substrate for cell microarray applications(A)(B)(C)(D)Figure 4.4. Fluorescence images of polymer arrays before (A and B) and after (C andD) overnight washing. For (A) and (C), from left to right each array setcorresponds to PAA, PEI, PLL and PVP printed onto a PEG coating at aninitial polymer concentration of 4 mg/mL. Across each row from left to rightin each array set the concentration of NSANB incubated with the polymerbefore spotting is altered between 2.0, 1.0, 0.5 and 0.0 mg/mL. Each columnis a set of three replicates. For (B) and (D), each array set is of PAAfunctionalised with NSANB printed onto a PEG coating at an initial polymerconcentration of 4 mg/mL. Across each row from left to right in each arrayset the concentration of NSANB incubated with the polymer before spottingis altered between 2.0, 1.0, 0.5 and 0.0 mg/mL. Each column is a set of threereplicates. The centre-to-centre distance between spots is 750 m.4-146