Patterned and switchable surfaces for biomaterial applications

Chapter 4 – Formation of a chemically patterned substrate for cell microarray applications(A)(B)Figure 4.2. Characterisation of the spotting of PEI onto an ALAPP-PEG surface. (A)1 mg/ml PEI spots spotted at varied humidity and temperature. From left toright, the first three spots correspond to humidity of 64%, 61% and 57%.The next six spots correspond to temperatures of 37 °C, 30 °C, 20 °C, 10 °C,5 °C and 3 °C. (B) 1 mg/ml PEI spots spotted at varied approach speed anddwell time. From left to right, the first three spots correspond to approachspeeds of 5 mm/s, 10 mm/s and 20 mm/s. The next seven spots correspond todwell times of 0 ms, 5 ms, 20 ms, 50 ms, 100 ms, 250 ms and 500 ms. Thespot diameter and the variability of pixel intensity for each spot is graphedunderneath each spot. The scale bars equal 500 µm.4-140