Patterned and switchable surfaces for biomaterial applications

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DNA (mg/m 2 )Chapter 3 – Comparison of the binding mode of plasmid DNA to allylamine plasma polymer and poly(ethyleneglycol) surfaces0.20.150.10.0500 10 20 30 40 50% ethanolFigure 3.8. DNA surface concentration after adsorption on ALAPP from aqueoussolutions with increasing % ethanol as detected by measurements of thedepletion of solution fluorescence. Each DNA adsorption experiment wasrepeated three times. Error reported to 95% confidence.3-124
Andrew Hook – Patterned and switchable surfaces for biomaterial applications3.3.4. Electro-stimulated DNA adsorption and desorptionAs a final study, the electro-stimulated adsorption and desorption of DNA to boththe ALAPP and PEG films was studied using measurements of the depletion ofsolution fluorescence. Initially the films were incubated in DNA solution overnight.Subsequently, +1.50 V was applied for 2 min to promote further DNA adsorption.After stabilisation for 4 mins, whereupon no voltage was applied, the polarity of thevoltage was reversed and -0.75 V was applied for 2 min in order to trigger DNAdesorption. After each voltage application, the DNA concentration of the bulksolution was determined by fluorescence spectroscopy and related to changes in the DNA . Delamination of the ALAPP or ALAPP-PEG films was not observed aftervoltage application.The results are shown as Figure 3.9. Notably, very low DNA (
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Patterned andswitchable surfacesfor
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TABLE OF CONTENTSTABLE OF CONTENTS
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CHAPTER 1.INTRODUCTION1The content
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Chapter 1 - Introductionconsiderabl
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Chapter 1 - Introductioninteraction
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Chapter 1 - IntroductionIn general,
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Chapter 1 - IntroductionFor some ap
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Chapter 1 - Introductionangles rang
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Chapter 1 - Introductionof understa
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Chapter 1 - IntroductionSeveral str
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Chapter 1 - Introductioncues to con
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Chapter 1 - Introductionby controll
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Chapter 1 - Introduction1.2.2. Soft
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Chapter 1 - Introductionsubstrate.
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Chapter 1 - Introduction(A)(B)Figur
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Chapter 1 - Introduction(A)(B)(C)(C
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Chapter 1 - Introductioncomplex gen
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Chapter 1 - Introductionmorphology
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Chapter 1 - Introduction(A)(B)(C)(D
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Chapter 1 - Introductionthe LCST, a
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Chapter 1 - IntroductionHyun et al.
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Chapter 1 - Introductionmicelles. T
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Chapter 1 - Introduction1.4.1. DNA
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Chapter 1 - IntroductionA major pro
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Chapter 1 - Introductionattached to
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Chapter 1 - IntroductionTable 1.1.
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Chapter 1 - Introductionefficiencie
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Chapter 1 - Introductionachieved by
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Chapter 1 - IntroductionTable 1.2.
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Chapter 1 - IntroductionOnce releas
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Chapter 1 - Introductionimaging not
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Chapter 1 - IntroductionA number of
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CHAPTER 2.SPATIALLY CONTROLLED ELEC
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Reflectivity (%) Reflectivity (%)An
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Reflectivity (%)SPR signal intensit
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SPR signal intensity (counts)Andrew
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Thickness (nm)Thickness (nm)Thickne
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CHAPTER 6.OVERALL CONCLUSIONS6.6An
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Chapter 6 - Overall conclusionsThes
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Chapter 6 - Overall conclusionsnew
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Force (nN)Force (nN)Force (nN)Force
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Patterned and switchable surfaces for biomaterial applications

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