Patterned and switchable surfaces for biomaterial applications

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Chapter 3 – Comparison of the binding mode of plasmid DNA to allylamine plasma polymer and poly(ethyleneglycol) surfacesconcentration of the adsorbed species, K is the equilibrium constant, s is thesurface concentration of the adsorbed species at saturation and C is the concentrationof the adsorbing species in the bulk solution. s KC s1 KC(3.3)Equation 3.3 was used to model the system and for the determination of values ofK and s using KaleidaGraph 4.0. Equation 3.3 can be rearranged to equation 3.4 byplotting the reciprocal of s against the reciprocal of C, whereupon a linear resultsuggests compliance with the Langmuir model [22].1 1 sK sC 1(3.4) s3.2.6. ElectrostimulatedDNA adsorption and desorptionQuantitative studies of DNA adsorption were conducted in a custom-builtelectrochemical cell as described previously [124]. ALAPP or PEG samples wereclamped into the cell and 2.4 ml of 100 ng/ml pEGFP-N1 solution in 0.01 Mphosphate buffer containing 1.0 M NaCl, pH = 7.4 was added over the sample. After24 hr incubation at room temperature, a voltage was applied in a stepwise fashion(+1.50 V, 0.00 V, -0.75 V) over consecutive 2 min intervals with the substrate as theworking electrode, an Ag/AgCl/saturated KCl reference electrode and a platinumauxiliary electrode. The voltages used were optimised previously on ALAPP films[34]. The DNA concentration of the solution was determined using the fluorescentPicogreen ® probe according to the manufacturer’s specifications before and aftereach voltage application. Solution removed for DNA concentration analysis wasreplaced with fresh PBS. Fluorescence measurements were performed using a LS 55Luminescence Spectrometer (Perkin Elmer Instruments).3-106
Andrew Hook – Patterned and switchable surfaces for biomaterial applicationsTable 3.1. Water contact angle measurements on bare silicon (Si), silicon modifiedwith ALAPP (Si-ALAPP) and silicon modified with ALAPP and grafted withPEG (Si-ALAPP-PEG). Error shown is to 95% confidence.Si Si-ALAPP Si-ALAPP-PEGContact angle 36° ±3° 70° ±2° 12° ±2°Table 3.2. Atomic concentration in % on silicon substrate materials as determinedby XPS. Value range shown to 95% confidence.C N O SiSi 11.6 ± 0.5 0.9 ± 0.2 36.0 ± 1.0 50.5 ± 1.3Si-ALAPP 76.5 ± 1.2 12.0 ± 0.6 11.5 ± 1.8 0.0 ± 0.0Si-ALAPP-PEG 70.0 ± 0.7 6.4 ± 0.3 23.6 ± 0.6 0.0 ± 0.03-107
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Patterned andswitchable surfacesfor
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TABLE OF CONTENTSTABLE OF CONTENTS
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CHAPTER 1.INTRODUCTION1The content
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Chapter 1 - Introductionconsiderabl
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Chapter 1 - Introductioninteraction
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Chapter 1 - IntroductionIn general,
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Chapter 1 - IntroductionFor some ap
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Chapter 1 - Introductionangles rang
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Chapter 1 - Introductionof understa
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Chapter 1 - IntroductionSeveral str
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Chapter 1 - Introductioncues to con
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Chapter 1 - Introductionby controll
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Chapter 1 - Introduction1.2.2. Soft
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Chapter 1 - Introductionsubstrate.
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Chapter 1 - Introduction(A)(B)Figur
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Chapter 1 - Introduction(A)(B)(C)(C
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Chapter 1 - Introductioncomplex gen
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Chapter 1 - Introductionmorphology
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Chapter 1 - Introduction(A)(B)(C)(D
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Chapter 1 - Introductionthe LCST, a
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Chapter 1 - IntroductionHyun et al.
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Chapter 1 - Introductionmicelles. T
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Chapter 1 - Introduction1.4.1. DNA
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Chapter 1 - IntroductionA major pro
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Chapter 1 - Introductionattached to
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Chapter 1 - IntroductionTable 1.1.
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Chapter 1 - Introductionefficiencie
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Chapter 1 - Introductionachieved by
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Chapter 1 - IntroductionTable 1.2.
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SPR signal intensity (counts)Andrew
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Reflectivity (%) Reflectivity (%)An
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Thickness (nm)Thickness (nm)Thickne
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CHAPTER 6.OVERALL CONCLUSIONS6.6An
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Force (nN)Force (nN)Force (nN)Force
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Patterned and switchable surfaces for biomaterial applications

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