Patterned and switchable surfaces for biomaterial applications

Andrew Hook – Patterned and switchable surfaces for biomaterial applicationsFigure 2.9. Fluorescence microscopy image of the formation of a TCM with HEK 293cells on an ALAPP/PEG patterned surface. Surfaces were patterned by laserablation and DNA was deposited onto spots by a robotic arrayer. Cells werestained with Hoechst 33342. The main image was taken through a 270-380nm excitation filter and a 410-580 nm suppression filter. The inset shows anoverlay of the Hoechst 33342 fluorescence and fluorescence due to theexpression of GFP, taken through a 450-490 nm excitation filter and a 515nm suppression filter. Scale bar is 150 m.2-93