Patterned and switchable surfaces for biomaterial applications

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Transfection efficiency (%)Transfection efficiency (%)Chapter 2 – Spatially controlled electro-stimulated DNA adsorption and desorption for biodeviceapplications(A)403020100-200-400-600-800-1000Voltage (mV)(B)403020100 1 2 3 4 5Time (min)Figure 2.8. Transfection efficiency of HEK 293 cells expressing pEGFP-N1 onALAPP substrate after 24 hr culture period; (A) with the application ofvarious voltage magnitudes for 2 mins, (B) with the application of -750 mVfor various lengths of time. Error bars are to 95% confidence.2-92
Andrew Hook – Patterned and switchable surfaces for biomaterial applicationsFigure 2.9. Fluorescence microscopy image of the formation of a TCM with HEK 293cells on an ALAPP/PEG patterned surface. Surfaces were patterned by laserablation and DNA was deposited onto spots by a robotic arrayer. Cells werestained with Hoechst 33342. The main image was taken through a 270-380nm excitation filter and a 410-580 nm suppression filter. The inset shows anoverlay of the Hoechst 33342 fluorescence and fluorescence due to theexpression of GFP, taken through a 450-490 nm excitation filter and a 515nm suppression filter. Scale bar is 150 m.2-93
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Patterned andswitchable surfacesfor
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TABLE OF CONTENTSTABLE OF CONTENTS
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Andrew Hook - Patterned and switcha
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CHAPTER 1.INTRODUCTION1The content
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Chapter 1 - Introductionconsiderabl
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Chapter 1 - Introductioninteraction
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Chapter 1 - IntroductionIn general,
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Chapter 1 - IntroductionFor some ap
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Chapter 1 - Introductionangles rang
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Chapter 1 - Introductionof understa
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Chapter 1 - IntroductionSeveral str
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Chapter 1 - Introductioncues to con
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Chapter 1 - Introductionby controll
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Chapter 1 - Introduction1.2.2. Soft
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Chapter 1 - Introductionsubstrate.
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Chapter 1 - Introduction(A)(B)Figur
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Chapter 1 - Introduction(A)(B)(C)(C
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Chapter 1 - Introductioncomplex gen
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Chapter 1 - Introductionmorphology
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Chapter 1 - Introduction(A)(B)(C)(D
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Chapter 1 - Introductionthe LCST, a
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Chapter 1 - IntroductionHyun et al.
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Chapter 1 - Introductionmicelles. T
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Chapter 4 - Formation of a chemical
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CHAPTER 5.SURFACE PLASMON RESONANCE
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SPR signal intensity (counts)Andrew
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Reflectivity (%) Reflectivity (%)An
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Reflectivity (%)SPR signal intensit
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SPR signal intensity (counts)Andrew
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Thickness (nm)Thickness (nm)Thickne
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CHAPTER 6.OVERALL CONCLUSIONS6.6An
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Chapter 6 - Overall conclusionsThes
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Force (nN)Force (nN)Force (nN)Force
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