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training module for medical officers primary health centre - NVBDCP

training module for medical officers primary health centre - NVBDCP

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Examination of blood films <strong>for</strong> malaria parasites (various blood stages of P. falciparum andP.vivax are shown in Plates 1 and 2 respectively)Examination of thick filmsExamine 100 fields, following the pattern of movement shown. To assist in examination, use a handtally counter to count fields. Record findings on the appropriate record <strong>for</strong>m. A parasite count may beincluded. Examine the blood film following the pattern of movement shown.Examination of thin filmsIt is usually in the distal third of the blood film that cells are: Most evenly distributed In a single layer and Have minimum distortionExamine the blood film following the pattern of movement shown. Examination of thin films may benecessary <strong>for</strong> confirmation of species identification or when thick films have been poorly prepared.Methods of counting malaria parasites in thick blood filmsIt is often necessary to determine the density of malaria parasites in thick blood films so that thephysician is aware of the severity of the infection and the parasites are responding to treatment. Twomethods are employed to count the parasites in thick blood films: Determination of parasites /l of blood Plus systemDetermination of parasites / ml of blood(a) If, after counting 200 leukocytes, 10 or more parasites are found, record the results in terms ofnumber of parasites / 200 leukocytes. Using a <strong>for</strong>mula, multiplying the number of parasites by 800026

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