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<strong>Column</strong>s for Special ApplicationsScale-Up from Analytical to Prep ZORBAX SB-C18 <strong>Column</strong>sUsing the Same Pump<strong>Column</strong> SizeFlow(mL/min)Injection(µL) Detector Cell Part No.<strong>Column</strong> 1 50 x 150 mm 100 2200 0.3 mm quartz Custom <strong>Column</strong><strong>Column</strong> 2 21.2 x 150 mm 18 400 0.3 mm quartz 877150-102<strong>Column</strong> 3 9.4 x 150 mm 3.5 80 0.3 mm quartz 883975-202<strong>Column</strong> 4 4.6 x 150 mm 0.85 2.0 3 mm SST 883975-902Scale-Up to PrepHT1. Theobromine35003000232. Theophylline3. CaffeineAbundance (mAU)25002000150010005000<strong>Column</strong> 11<strong>Column</strong> 2<strong>Column</strong> 3<strong>Column</strong> 40 2.5 5.0 7.5 10.0 12.5 15.0 17.5 20.0Time (min)LCPHT_ScaleUpUsing the same 1100 pump, a scale-up from 4.6 mm to 50 mm ID was possible without any loss ofresolution. This increases throughput by reducing the time required for redeveloping and adjustingthe method.96 Order online at www.agilent.com/chem/store