Agilent HPLC Column Selection Guide
Agilent HPLC Column Selection Guide Agilent HPLC Column Selection Guide
Columns for Special ApplicationsZORBAX PrepHT• Easy scale-up from analytical to preparative scale with ZORBAX phases• Fast preparative separations, up to 2000 mg• 5 to 7 µm particles for high efficiency and high yield• Easy to install finger tight connections seal up to 5000 psi/350 barHigh purity, high recovery and high throughput can be easily achieved with AgilentZORBAX PrepHT columns. These are available in a variety of bonded phases – Eclipse XDB,StableBond, Bonus-RP, and Extend-C18 – for optimized resolution and loadability underany conditions.ZORBAX PrepHT columns are packed with 5 and 7 µm particle sizes for very high resolution.The high resolution allows high loadability, high yield, and high purity of compounds. Thelarger diameter columns and mechanically stronger ZORBAX particles allow for flow rates upto 100 ml/min, thus increasing throughput.ZORBAX PrepHT columns are designed for rapid scale-up from analytical to preparative scalewithout losing resolution. For complex separations on larger columns (21.2 mm ID, 150 mmlength and longer), Agilent has carefully chosen the 7 µm particle size to achieve a balancebetween high efficiency and high loadability.94 Order online at www.agilent.com/chem/store
Columns for Special ApplicationsHigh Purity and High Recovery with ZORBAX PrepHT ColumnsSample:Antianginal drugsMass-based fraction collection using ZORBAXSB-C18 column shows high purity and highrecovery of each compound (Application Notepublication number 5988-7113EN). Theseparation of the three antianginal drugs wassuccessfully done in a single run with highrecovery and >90% purity. Separations up to2000 mg are possible depending on thecomplexity of separation.Abundance (mAU)DMSO1. Nifedipine2. Nimodine3. Nisoldipine123DAD80000000 1 2 3 4 5 6 7Time (min)Vial 2 – 343Vial 1 – 347Vial 3 – 389MSD6000000400000020000000 1 2 3 4 5 6 7Time (min)LCPHT01AmountNifedipin [mg]AmountNifmodipin [mg]AmountNifsoldipin [mg]Fraction 1 18.90 0.11 0.16 Purity Nifedipin 98.6%Fraction 2 0.29 17.66 0.77 Purity Nifmodipin 94.4%Fraction 3 0.49 1.66 18.36 Purity Nifsoldipin 89.5%Recovery [mg] 19.68 19.43 19.29Recovery [%] 101.3 102.0 101.9Order online at www.agilent.com/chem/store95
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<strong>Column</strong>s for Special ApplicationsHigh Purity and High Recovery with ZORBAX PrepHT <strong>Column</strong>sSample:Antianginal drugsMass-based fraction collection using ZORBAXSB-C18 column shows high purity and highrecovery of each compound (Application Notepublication number 5988-7113EN). Theseparation of the three antianginal drugs wassuccessfully done in a single run with highrecovery and >90% purity. Separations up to2000 mg are possible depending on thecomplexity of separation.Abundance (mAU)DMSO1. Nifedipine2. Nimodine3. Nisoldipine123DAD80000000 1 2 3 4 5 6 7Time (min)Vial 2 – 343Vial 1 – 347Vial 3 – 389MSD6000000400000020000000 1 2 3 4 5 6 7Time (min)LCPHT01AmountNifedipin [mg]AmountNifmodipin [mg]AmountNifsoldipin [mg]Fraction 1 18.90 0.11 0.16 Purity Nifedipin 98.6%Fraction 2 0.29 17.66 0.77 Purity Nifmodipin 94.4%Fraction 3 0.49 1.66 18.36 Purity Nifsoldipin 89.5%Recovery [mg] 19.68 19.43 19.29Recovery [%] 101.3 102.0 101.9Order online at www.agilent.com/chem/store95
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