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<strong>Column</strong>s for Special ApplicationsCombine RRHT and Solvent Saver for Maximum SolventReduction: Diet Soda<strong>Column</strong> A: Eclipse Plus C18959993-9024.6 x 150 mm, 5 µmF = 1.0 mL/min (P = 96 bar)1. Saccharine<strong>Column</strong> B: Solvent Saver HT, 600 bar2. CaffeineEclipse Plus C18959941-302A 1 11 mL/analysis 3. Aspartame3.0 x 50 mm, 1.8 µm4. Benzoic acidF = 0.43 (P = 181 bar)2Mobile Phase: A: 20 mM Phosphate34B: AcetonitrileA:B (79:21)2 4 6 8Temperature: 25°CDetector: UV 220 nm, 16, ref = 420, 20 B1.6 mL/analysisFlow Cell: 3 mm, 2 µLFlow Rate: 1 sInj. Vol 3 µL for 4.6, 1.5 for 3.0Instrument: 1200SL2 3 4Sample:0.2 µm filtered diet soda1085% less solventminRRHT_dietsodaSolvent Saver <strong>Column</strong>s are Ideal for LC/MS<strong>Column</strong>: ZORBAX SB-C18861954-3023.0 x 100 mm, 3.5 µmMobile Phase: A: 70% Methanol+0.4% Formic AcidB: 30% Water+0.4% Formic AcidFlow Rate: 0.425 mL/minDetector: A: UV 254 nmB: Positive Ion APCIC: Positive Ion ElectrospraySample:SteroidsSolvent Saver columns are ideal for LC/MS because the typical0.5 mL/min flow rate allows samples to be evaluated andanalyzed without changing columns when the MS interface ischanged from electrospray to APCI.mAU14012010080604020035000000300000002500000020000000150000001000000050000000A123450 2 4 6 8 10 12 14 16Time (min)BLC-UVLC-APCI-MS1. Triamcinolone2. Hydrocortisone3. Cortisone acetate4. Deoxycorticosterone5. Hydroxyprogesterone6. Progesterone0 2 4 6 8 10 12 14 16Time (min)6CLC-ESI-MS25000000225000002000000017500000150000001000000075000000 2 4 6 8 10 12 14 16Time (min)LCSS003Order online at www.agilent.com/chem/store91
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