Agilent HPLC Column Selection Guide

Columns for Special ApplicationsZORBAX Solvent Saver• Provide 60% reduction in mobile phase usage and waste generation comparedto a 4.6 mm ID column• Provide 2- to 3-fold signal-to-noise (S/N ratio) improvement• Deliver optimal LC/MS performance at intermediate flow rates• Can be used with most conventional LC instrument configurations without modification• Solvent Saver columns are available in 1.8, 3.5 and 5 µm particle sizesAgilent ZORBAX Solvent Saver columns have a 3.0 mm internal diameter. This is ideal forreducing solvent usage by 50% over 4.6 mm ID columns. Solvent Saver columns are alsoideal for LC/MS. With a typical flow rate of 0.5 ml/min, these columns are compatible withalmost all LC interfaces. These columns also improve sensitivity 2 to 3 times over 4.6 mm IDcolumns. Solvent Saver columns can be used with conventional HPLC instruments and area good choice for cost effective analyses.Solvent Saver Columns Provide up to 60% Reductionin Solvent Use and WasteColumn A:Column B:ZORBAX SB-C18883975-9024.6 x 150 mm, 5 µmZORBAX SB-C18883975-3023.0 x 150 mm, 5 µmMobile Phase: 20% ACN: 80% 0.2 M Na 2 HPO 4+ 0.1 M Citric Acid, pH 2.6Temperature: AmbientSample:AntibacterialsA1Less solventconsumption, less wasteB11. Sulfamerazine2. Furazolidone3. Oxolinic Acid4. Sulfadimethoxine5. Sulfaquinoxaline6. Nalidixic AcidThis separation of antibacterials on a 4.6 and3.0 mm ID columns, shows that solvent useis reduced by 50% just by changing to theSolvent Saver column with no change inthe chromatography. This reduces the costof analyses dramatically.23456234560Time (min)400Time (min)40LCSS00190 Order online at www.agilent.com/chem/store