13.07.2015 Views

Agilent HPLC Column Selection Guide

Agilent HPLC Column Selection Guide

Agilent HPLC Column Selection Guide

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<strong>Column</strong>s for Special ApplicationsZORBAX Rapid Resolution 3.5 µm• Ideal choice for initial method development• Reduce analysis time and solvent usage and increase sample throughput• High efficiency in short and ultra-short column lengths• Available in analytical (4.6 mm) and narrow-bore (2.1 mm) ID• Comparable lifetime to 5 µm columns<strong>Agilent</strong> ZORBAX Rapid Resolution columns, with a 3.5 µm particle size, reduce analysistime and increase sample throughput for any application when compared to 5 µm columns.Rapid Resolution columns are available from 15-150 mm, in 1-4.6 mm IDs, so the bestconfigurations are available for high throughput, LC/MS, combinatorial chemistry and rapidanalytical applications. Rapid Resolution 3.5 µm particles have superior mechanical strength,so every column has an extremely stable packed bed and provides a comparable lifetimeto 5 µm columns. These are available in most ZORBAX bonded phases.Rapid Resolution <strong>Column</strong>s Reduce Analysis TimeWhile Maintaining Resolution<strong>Column</strong> A: ZORBAX Bonus-RP883668-9014.6 x 150 mm, 5 µm<strong>Column</strong> B: ZORBAX Bonus-RP866668-9014.6 x 75 mm, 3.5 µm<strong>Column</strong> C: ZORBAX Bonus-RP861700-9012.1 x 50 mm, 3.5 µmMobile Phase: 80% 25 mM NH 4 Ac, pH 5.520% ACNFlow Rate: 1.5 mL/min1.5 mL/min0.3 mL/minTemperature: 40°CDetector: 254 nm1. Caffeine2. Doxylamine3. ChlorpheniramineA1 4. Triprolidine4230 1 2 3 4 5 6Time (min)B 1C1422334Rapid Resolution 3.5 µm columns are available in narrow bore configurations forgreat compatibility with LC/MS.01 2 3Time (min)01 2Time (min)LC3_501Order online at www.agilent.com/chem/store85

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