Agilent HPLC Column Selection Guide
Agilent HPLC Column Selection Guide Agilent HPLC Column Selection Guide
Columns for Special ApplicationsReduce Analysis Time Dramaticallywith Rapid Resolution HT ColumnsColumn A: Eclipse XDB-C18990967-9024.6 x 250 mm, 5 µmColumn B: Eclipse XDB-C18963967-9024.6 x 150 mm, 3.5 µmColumn C: Eclipse XDB-C18966967-9024.6 x 75 mm, 3.5 µmColumn D: ZORBAX Eclipse XDB-C18935967-9024.6 x 50 mm, 3.5 µmColumn E: Eclipse XDB-C18925975-9024.6 x 50 mm, 1.8 µmMobile Phase: 73% MeOH: 27% 20 mMPhosphate Buffer, pH 7.0Flow Rate: 1 mL/minTemperature: AmbientDetector: 254 nmA12 34567BCDE11231 52 3 41 523 4254667736745671. Uracil2. Naproxen3. Mefanamic Acid4. Butyl Paraben5. Propranolol6. Napthalene7. Dipropyl Phthalate0 2 4 6 8 1012Time (min)LCRR003This figure shows the dramatic reduction in analysis time possible by using Rapid Resolution HT columns. Chromatogram A shows a separation that takes 11.5 minuteson a 25 cm, 5 µm column. Rapid Resolution (3.5 µm) columns, shown in chromatogram B and C, reduce analysis time substantially, but with a slight compromise inresolution. The Rapid Resolution HT column reduces analysis time to 2.2 minutes, an 80% reduction, while still maintaining baseline resolution.80 Order online at www.agilent.com/chem/store
Columns for Special ApplicationsLong Lifetime of RRHT Columns at Elevated TemperaturesColumn: ZORBAX SB-C18827700-9022.1 x 50 mm, 1.8 µmMobile Phase: A: 60% H 2 OB: 40% ACNFlow Rate: 1 mL/minTemperature: 80°CDetector: UV 254 nmSample:QC Test MixPlates1200010000800060004000200001499718853988115313051506164117761911204621812319250826432778291330853220346836033738388740544189432445374756489250335201Injection NumberLCRR005Comparison of Efficiencies – RRHT (1.8 µm)and Rapid Resolution (3.5 µm) ColumnsColumn LengthResolving PowerN (3.5 µm)*Resolving PowerN (1.8 µm)High Resolution150 21,000 32,500100 14,000 24,00075 10,500 17,000**Ultra Fast50 7000 12,00030 4200 600020 — 350015 2100 2500Resolution α N 1/2*5 µm HPLC columns of the same length have 40% fewer plates (N-value); 4.6 mm ID**Available as a custom columnOrder online at www.agilent.com/chem/store81
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<strong>Column</strong>s for Special ApplicationsReduce Analysis Time Dramaticallywith Rapid Resolution HT <strong>Column</strong>s<strong>Column</strong> A: Eclipse XDB-C18990967-9024.6 x 250 mm, 5 µm<strong>Column</strong> B: Eclipse XDB-C18963967-9024.6 x 150 mm, 3.5 µm<strong>Column</strong> C: Eclipse XDB-C18966967-9024.6 x 75 mm, 3.5 µm<strong>Column</strong> D: ZORBAX Eclipse XDB-C18935967-9024.6 x 50 mm, 3.5 µm<strong>Column</strong> E: Eclipse XDB-C18925975-9024.6 x 50 mm, 1.8 µmMobile Phase: 73% MeOH: 27% 20 mMPhosphate Buffer, pH 7.0Flow Rate: 1 mL/minTemperature: AmbientDetector: 254 nmA12 34567BCDE11231 52 3 41 523 4254667736745671. Uracil2. Naproxen3. Mefanamic Acid4. Butyl Paraben5. Propranolol6. Napthalene7. Dipropyl Phthalate0 2 4 6 8 1012Time (min)LCRR003This figure shows the dramatic reduction in analysis time possible by using Rapid Resolution HT columns. Chromatogram A shows a separation that takes 11.5 minuteson a 25 cm, 5 µm column. Rapid Resolution (3.5 µm) columns, shown in chromatogram B and C, reduce analysis time substantially, but with a slight compromise inresolution. The Rapid Resolution HT column reduces analysis time to 2.2 minutes, an 80% reduction, while still maintaining baseline resolution.80 Order online at www.agilent.com/chem/store
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