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<strong>Column</strong>s for Special ApplicationsRapid Resolution HT Provides Doublethe Efficiency of Rapid Resolution <strong>Column</strong>s<strong>Column</strong> A:<strong>Column</strong> B:Mobile Phase:Flow Rate:Temperature:Detector:ZORBAX SB-C18835975-9024.6 x 50 mm, 3.5 µmZORBAX SB-C18827975-9024.6 x 50 mm, 1.8 µm25% Water, 75% MeOH1.5 mL/minAmbient254 nmThis figure shows that Rapid Resolution HTcolumns can provide double the efficiencyof a 3.5 µm column in the same column length.This high efficiency can be used for veryhigh-resolution, high throughput analyses.A012Plates (N)1. 34762. 45853. 56734. 61803Time (min)41B1. Uracil2. Phenol3. 4-CI-Nitrobenzene4. Toluene0.012Plates (N)1. 65602. 89583. 115084. 1226630.5Time (min)41.0LCRR002Increase Peak Capacity with RRHT <strong>Column</strong>s<strong>Column</strong> A: Eclipse XDB-C8928700-9062.1 x 100 mm, 1.8 µm<strong>Column</strong> B: Eclipse XDB-C18961753-9022.1 x 100 mm, 3.5 µmMobile Phase: A: H 2 OB: ACNPeak capacity: A: 461B: 343Flow Rate: 0.5 mL/minGradient: 0.0 min 50% B20.0 min 100% BTemperature: 40°CDetector: UV 254 nmSample:AlkylphenonesAmAU120100806040200BmAU100806040200110 2 4 6 8 10 12 14 16 18Time (min)11. Uracil2. C 3 -Alkylphenone3. C 4 -Alkylphenone4. C 5 -Alkylphenone5. C 6 -Alkylphenone0 2 4 6 8 10 12 14 16 18Time (min)116. C 7 -Alkylphenone7. C 8 -Alkylphenone8. C 9 -Alkylphenone9. C 10 -Alkylphenone10. C 12 -Alkylphenone11. C 14 -Alkylphenone11LCRR004Order online at www.agilent.com/chem/store79
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