Agilent HPLC Column Selection Guide

Columns for Special Applications1100 Series Conversion Kits for Fast LCThese kits make it easy to convert your Agilent 1100 system with a binary pump to alower-volume system for RRHT LC columns. Each kit contains all capillaries, a flow cell,starter columns, and detailed instructions for system conversion. Note: you will still be ableto use your converted 1100 for standard methods and columns.Kit Selection Description Part No.For Variable WavelengthDetectors (VWD)For Diode Array Detectors(DAD & DAD SL) and MultipleWavelength Detectors (MWD)For Diode Array Detectorand Mass SpecColumns: 4.6 x 50 mm, 1.8 µm (3)Flow Cell for VWD, 5 µl capillaries, µ-LC inline filterColumns: 4.6 x 50 mm, 1.8 µm (2)Flow Cell for DAD, 5 µl capillaries, µ-LC inline filterColumns: 2.1 x 50 mm, 1.8 µm (2)Flow Cell for DAD, 1.7 µl capillaries, ZDV union5188-53235188-53245188-5328Rapid Resolution HT: Up to 20X FasterColumn A: ZORBAX SB-C18883975-9024.6 x 150 mm, 5 µmColumn B: ZORBAX SB-C18827700-9022.1 x 50 mm, 1.8 µmColumn C: ZORBAX SB-C18827700-9022.1 x 50 mm, 1.8 µmMobile Phase: A: H 2 OB: ACNGradient: 0.0 min 50% BA: 11 min 100%BB: 1.2 min 100%BC: 0.4 min 100%BFlow Rate: A: 1.2 mL/minB: 1.0 mL/minC: 2.4 mL/minTemperature: A: 40°CB: 40°CC: 95°CDetector: UV 254 nmSample:Alkylphenones11. C 3 -AlkylphenoneA2435 67 8 92.3.C 4 -AlkylphenoneC 5 -Alkylphenone4. C 6 -Alkylphenone5. C 7 -Alkylphenone40 2 4 6 8 10Time (min)6.7.C 8 -AlkylphenoneC 9 -AlkylphenoneB128. C 10 -Alkylphenone35 69. C 12 -Alkylphenone7 8 90 0.2 0.4 0.6 0.8 1.0Time (min)41 2C35 67 8 90 0.2 0.4Time (min)LCRR00178 Order online at www.agilent.com/chem/store