Agilent HPLC Column Selection Guide
Agilent HPLC Column Selection Guide Agilent HPLC Column Selection Guide
Columns for Special ApplicationsSub 1 Minute Separations with RRHD ColumnsColumn:Gradient:ZORBAX RRHD SB-C18857700-9022.1 x 50 mm, 1.8 µmH 2 O (0.05% trifluoroacetic acid)/10-40% ACN/1minTemperature: 60°CInjection Volume:Detector Wavelength:Data Rate:0.5 µL x 100 ppm each275 nm160 HzmAU350040030016 Compound Biocide Mix in 0.5 minF = 2.0 mL/minP = 975 bar200100026540 .05 .1 .15 .2 .25 .3 .35 .4 .450.5 minmin1. 2-methyl-4-isothiazolin-3-one2. 5-chloro-2-methyl-4-isothiazolin-3-one3. Carbendazim4. Benzisothiiazol-3(2H)-one5. 2-phenoxyethanol6. MethylparabenRRHDSub1mRapid Resolution HD Columns for High Pressure Use(Maximum Pressure: 1200 bar)DescriptionSolvent Saver HD,1200 barSolvent Saver HD,1200 barNarrow Bore RRHD,1200 barNarrow Bore RRHD,1200 barNarrow Bore RRHD,1200 barSize (mm)ParticleSize (µm)EclipsePlus C18USP L1SB-C18USP L13.0 x 100 1.8 959758-302 858700-3023.0 x 50 1.8 959757-302 857700-302SB-C8USP L72.1 x 150 1.8 959759-902 859700-902 859700-9062.1 x 100 1.8 959758-902 858700-902 858700-9062.1 x 50 1.8 959757-902 857700-902 857700-90676 Order online at www.agilent.com/chem/store
Columns for Special ApplicationsZORBAX Rapid ResolutionHigh Throughput 1.8 µm• High pressure (600 bar) columns for ultra high speed or maximum resolution analyseswith Rapid Resolution HT columns packed with totally porous, 1.8 µm packings• Carefully engineered particles deliver maximum resolution at 25% less pressure thanother sub 2-micron materials• Reduce analysis time by up to 95%• Develop HPLC methods more quickly• Securely transfer conventional methods with over 140 RRHT column choices• Analyze complex samples on shorter columns faster and maximize peak capacity• Matching selectivity in 3.5, 5 and 7 µm particle sizes for complete method scalability• Short (50 mm long and less) column can be used on some conventional LCsAgilent ZORBAX Rapid Resolution HT (1.8 µm) columns use a totally porous, 1.8 µm particleto provide maximum resolution in fast, ultra-fast and high resolution analyses. You can reduceanalysis time by up to 95% in comparison to 250 mm length columns. With more than140 RRHT column choices, including the new high performance ZORBAX Eclipse Plus andmany other ZORBAX column choices (Eclipse XDB, StableBond, Extend, Bonus-RP), methodscan be developed quickly or securely transferred to a smaller particle size column with noloss in resolution. The small particle size provides double the efficiency of a 3.5 µm columnin the same column length providing the highest efficiency and resolution possible. Thispermits the analysis of complex samples on shorter column with the highest resolution andpeak capacity. The 1.8 µm Rapid Resolution HT columns take high-speed, high-resolutionHPLC to a new level.The 600 bar columns can be used with the Agilent 1200 Rapid Resolution LC up to this highpressure limit. In addition, the shorter columns can be used on many other LC's, including theAgilent 1200 and 1100 by using the RRHT-1100 conversion kits to maximize performance.Order online at www.agilent.com/chem/store77
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<strong>Column</strong>s for Special ApplicationsZORBAX Rapid ResolutionHigh Throughput 1.8 µm• High pressure (600 bar) columns for ultra high speed or maximum resolution analyseswith Rapid Resolution HT columns packed with totally porous, 1.8 µm packings• Carefully engineered particles deliver maximum resolution at 25% less pressure thanother sub 2-micron materials• Reduce analysis time by up to 95%• Develop <strong>HPLC</strong> methods more quickly• Securely transfer conventional methods with over 140 RRHT column choices• Analyze complex samples on shorter columns faster and maximize peak capacity• Matching selectivity in 3.5, 5 and 7 µm particle sizes for complete method scalability• Short (50 mm long and less) column can be used on some conventional LCs<strong>Agilent</strong> ZORBAX Rapid Resolution HT (1.8 µm) columns use a totally porous, 1.8 µm particleto provide maximum resolution in fast, ultra-fast and high resolution analyses. You can reduceanalysis time by up to 95% in comparison to 250 mm length columns. With more than140 RRHT column choices, including the new high performance ZORBAX Eclipse Plus andmany other ZORBAX column choices (Eclipse XDB, StableBond, Extend, Bonus-RP), methodscan be developed quickly or securely transferred to a smaller particle size column with noloss in resolution. The small particle size provides double the efficiency of a 3.5 µm columnin the same column length providing the highest efficiency and resolution possible. Thispermits the analysis of complex samples on shorter column with the highest resolution andpeak capacity. The 1.8 µm Rapid Resolution HT columns take high-speed, high-resolution<strong>HPLC</strong> to a new level.The 600 bar columns can be used with the <strong>Agilent</strong> 1200 Rapid Resolution LC up to this highpressure limit. In addition, the shorter columns can be used on many other LC's, including the<strong>Agilent</strong> 1200 and 1100 by using the RRHT-1100 conversion kits to maximize performance.Order online at www.agilent.com/chem/store77