13.07.2015 Views

Agilent HPLC Column Selection Guide

Agilent HPLC Column Selection Guide

Agilent HPLC Column Selection Guide

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<strong>Column</strong>s for Special ApplicationsSeparation of Licorice Root on RRHD <strong>Column</strong>s<strong>Column</strong> A: ZORBAX RRHD SB-C18857700-9022.1 x 50 mm, 1.8 µm<strong>Column</strong> B: 858700-9022.1 x 100 mm, 1.8 µm<strong>Column</strong> C: 859700-9022.1 x 150 mm, 1.8 µmMobile Phase:Flow Rate:Gradient:Temperature:Detector:Instrument:10-100% B /30 minA: 0.1% formic acid (fa)B: acetonitrile with 0.1% faF = 0.4 mL/min30 minute gradient on each lengthAmbient280 nm UV1290 Infinity LCAmAU20015010050007 peaks50 mmRs: 0242 4 6 8 10 12 14 16 18 min100 mmB8 peaksRs: 1.37mAU20015010050002 4 6 8 10 12 14 16 1824min150 mmC9 peaksRs: 2.40mAU20015010050004 62 4 6 8 10 12 14 16 182minRRHD_LicoriceOrder online at www.agilent.com/chem/store75

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