Agilent HPLC Column Selection Guide
Agilent HPLC Column Selection Guide Agilent HPLC Column Selection Guide
Columns for Analytical HPLCZORBAX Method Validation KitsZORBAX Method Validation Kits are supplied to customers who need the same HPLCcolumn type (bonded phase, particle size, configuration) but from different manufacturinglots. To request columns from different lots, contact Agilent Technologies or your localAgilent Authorized Distributor using the following procedure.• Request Validation Kits (columns from different lots) by using Part Number 899999-888• Indicate the Part Number of the current column you are using• Indicate the Lot Number of the current column you are using• Indicate the number of additional columns needed from different lots (example: you havea current column and may need two additional lots)• Please fax your request to (302) 993-5354 or email to custom_columns@agilent.com.You will receive a quote from your Customer Service Agent within 1-2 business days.Delivery for your custom column is usually 3 weeks or less from the time your order isplaced, depending on lot availability.Custom HPLC Column OrderingColumns not listed can easily be ordered by the following procedure:Tips & ToolsRequest custom LC columns online atwww.agilent.com/chem/customlccol• Request a Special Products Quotation (SPQ) using Part Number 899999-999• Indicate column dimensions (example: 4.6 x 50 mm); bonded phase type (example:StableBond C3); particle size (example: 5 µm); and pore size (example: 80Å)• Please fax your request to (302) 993-5354 or email to custom_columns@agilent.com.You will receive a quote from your Customer Service Agent within 1-2 business days.Delivery for your custom column is usually 3 weeks or less from the time your order isplaced, depending on lot availability.Custom columns are priced with a minimal surcharge over the price of stocked columns.70 Order online at www.agilent.com/chem/store
Columns for Analytical HPLCZORBAX Ion Exchange Columns –SAX and SCX• ZORBAX SAX and 300SCX columns are based on rugged ZORBAX silica• Stable from pH 2-7• Provide high efficiency, rapid separations• Compatible with organic mobile phase modifiersAgilent ZORBAX Strong Ion Exchange columns are available as both Strong Anion Exchange(SAX) and Strong Cation Exchange (300SCX) columns. Each column is packed with bonded,5 µm, spherical silica particles for optimum efficiency.ZORBAX SAX packing has a permanently bonded quaternary amine. A trifunctional organosilanereagent is used in producing this packing to maximize its stability with aqueous mobilephases. This column is ideal for separation of water-soluble compounds such as aromaticand aliphatic carboxylic acids and sulfonic acids.ZORBAX SCX packing has 300Å pore size silica particles chemically bonded to an aromaticsulfonic acid group. This column is used for separations of basic, water-soluble compoundsand bio-molecules.Column SpecificationsBondedPhase Pore Size Surface Area pH Range FunctionalityMaxPressureZORBAX SAX 70Å 300 m 2 /g 2.0-7.0 Quaternary 350 baramineZORBAX300SCX300Å 50 m 2 /g 2.0-7.0 Sulfonic acid 350 barSpecifications represent typical values only.Order online at www.agilent.com/chem/store71
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<strong>Column</strong>s for Analytical <strong>HPLC</strong>ZORBAX Method Validation KitsZORBAX Method Validation Kits are supplied to customers who need the same <strong>HPLC</strong>column type (bonded phase, particle size, configuration) but from different manufacturinglots. To request columns from different lots, contact <strong>Agilent</strong> Technologies or your local<strong>Agilent</strong> Authorized Distributor using the following procedure.• Request Validation Kits (columns from different lots) by using Part Number 899999-888• Indicate the Part Number of the current column you are using• Indicate the Lot Number of the current column you are using• Indicate the number of additional columns needed from different lots (example: you havea current column and may need two additional lots)• Please fax your request to (302) 993-5354 or email to custom_columns@agilent.com.You will receive a quote from your Customer Service Agent within 1-2 business days.Delivery for your custom column is usually 3 weeks or less from the time your order isplaced, depending on lot availability.Custom <strong>HPLC</strong> <strong>Column</strong> Ordering<strong>Column</strong>s not listed can easily be ordered by the following procedure:Tips & ToolsRequest custom LC columns online atwww.agilent.com/chem/customlccol• Request a Special Products Quotation (SPQ) using Part Number 899999-999• Indicate column dimensions (example: 4.6 x 50 mm); bonded phase type (example:StableBond C3); particle size (example: 5 µm); and pore size (example: 80Å)• Please fax your request to (302) 993-5354 or email to custom_columns@agilent.com.You will receive a quote from your Customer Service Agent within 1-2 business days.Delivery for your custom column is usually 3 weeks or less from the time your order isplaced, depending on lot availability.Custom columns are priced with a minimal surcharge over the price of stocked columns.70 Order online at www.agilent.com/chem/store