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<strong>Column</strong>s for Analytical <strong>HPLC</strong>Separation of Group 4 Analytes in EPA 1694on ZORBAX HILIC Plus <strong>Column</strong><strong>Column</strong>: ZORBAX HILIC Plus959793-9012.1 x 100 mm, 3.5 µmMobile Phase: 90% Acetontrile:10% WaterFlow Rate: 0.25 mL/minGradient: Linear gradient to 55% acetonitrilein 7 min Held at 55%Temperature: 25°Cx10 21.000.950.900.850.800.750.700.650.600.550.500.450.400.350.300.250.200.150.100.050CimetidineAlbuterolRanitidineMetforminx10 2 671.000.950.900.850.800.750.700.650.600.550.500.450.400.350.300.250.200.150.100.0500.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 6.5 7.0 7.5 8.0 8.5 9.0 9.5 10.0 10.5 11.0 11.5 12.0 12.5 13.0 13.5 14.0 14.5 15.0 15.5 16.0 16.5 17.0 17.5 18.0 18.5 19.0 19.5 20.0 20.5 21.0 21.5 22.0 22.5 23.0 23.5 24.0 24.5Duplicate runs for column USCJP0004 10 min equilibration between two runsEPA1694ZORBAX HILIC PlusDescription Size (mm) Particle Size (µm) Part No.ZORBAX HILIC Plus 4.6 x 100 3.5 959961-901ZORBAX HILIC Plus 4.6 x 50 3.5 959943-901ZORBAX HILIC Plus 2.1 x 100 3.5 959793-901ZORBAX HILIC Plus 2.1 x 50 3.5 959743-901Order online at www.agilent.com/chem/store