13.07.2015 Views

Agilent HPLC Column Selection Guide

Agilent HPLC Column Selection Guide

Agilent HPLC Column Selection Guide

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<strong>Column</strong>s for Analytical <strong>HPLC</strong>ZORBAX HILIC Plus• HILIC column for good retention of small, polar analytes• Based on Eclipse Plus silica for excellent peak shape• High sensitivity for LC/MS applications• Recommended for EPA Method 1694<strong>Agilent</strong> ZORBAX HILIC Plus columns are for use in hydrophilic interaction chromatography(HILIC) applications. HILIC is typically used for the retention and resolution of small polarcompounds. HILIC Plus columns are non-bonded silica columns based on the highperformance silica used in the ZORBAX Eclipse Plus columns. This silica provides excellentpeak shape; critical for many polar, basic analytes. These columns ship prepared for use inHILIC mode – containing acetonitrile:water – in order to reduce the extensive equilibrationtypically required for HILIC separations. The HILIC Plus columns are available in a 3.5 µmparticle size for high resolution and in 2.1 and 4.6 mm ID for compatibility with massspectrometers or with standard UV detectors.<strong>Column</strong> SpecificationsBonded Phase Pore Size Surface Area pH RangeNon-bonded silica 95Å 160 m 2 /g 0-8.0Specifications represent typical values only.66 Order online at www.agilent.com/chem/store

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