Agilent HPLC Column Selection Guide
Agilent HPLC Column Selection Guide Agilent HPLC Column Selection Guide
Columns for Analytical HPLCNormal Phase Columns Based on ZORBAX Rx-SILHardware Description Size (mm) Particle Size (µm)Rx-SILUSP L3EclipseXDB-CNUSP L10Standard Columns (no special hardware required)Semi-Prep 9.4 x 250 5 880975-201Analytical 4.6 x 250 5 880975-901 990967-905*Analytical 4.6 x 150 5 883975-901 993967-905*Rapid Resolution HT, 600 bar 4.6 x 100 1.8 828975-901Rapid Resolution HT, 600 bar 4.6 x 50 1.8 827975-902Rapid Resolution HT, 600 bar 3.0 x 100 1.8 828975-301Rapid Resolution HT, 600 bar 3.0 x 50 1.8 827975-301Narrow Bore 2.1 x 150 5 883700-901 993700-905*Rapid Resolution HT, 600 bar 2.1 x 100 1.8 828700-901Rapid Resolution HT, 600 bar 2.1 x 50 1.8 827700-901Guard Columns (hardware required)Guard Cartridge, 2/pk 9.4 x 15 5 820675-119Guard Cartridges, 4/pk 4.6 x 12.5 5 820950-919 820950-935Guard Cartridge, 4/pk 2.1 x 12.5 5 821125-919 821125-935Guard Hardware Kit 840140-901Guard Hardware Kit 820888-901 820888-901PrepHT Cartridge Columns (require endfittings kit 820400-901)PrepHT Cartridge 21.2 x 250 7 877250-101PrepHT Cartridge 21.2 x 250 7PrepHT endfittings, 2/pk 820400-901PrepHT Guard Cartridge, 2/pk 17 x 7.5 5 820212-919Guard Cartridge Hardware 820444-901*These columns ship containing reversed phase solvents. Flush with isopropanol before using normal phase solvents.64 Order online at www.agilent.com/chem/store
Columns for Analytical HPLCNormal Phase Columns Based on ZORBAX Original SILHardware DescriptionSize (mm)Particle Size(µm)SILUSP L3CNUSP L10NH2USP L8CarbohydrateAnalysisStandard Columns (no special hardware required)Semi-Prep 9.4 x 250 5 880952-201 880952-205 880952-208Analytical 4.6 x 250 5 880952-701 880952-705 880952-708 840300-908*Analytical 4.6 x 150 5 883952-701 883952-705 883952-708 843300-908*Narrow Bore 2.1 x 50 5 860700-708Guard Columns (hardware required)Guard Cartridge, 2/pk 9.4 x 15 5 820675-119 820675-111 820675-111Guard Cartridges, 4/pk 4.6 x 12.5 5 820950-901 820950-905 820950-908 820950-908*Guard Cartridge, 4/pk 2.1 x 12.5 5Guard Hardware Kit 840140-901 840140-901 840140-901Guard Hardware Kit 820888-901 820888-901 820888-901 820888-901*PrepHT Cartridge Columns (require endfittings kit 820400-901)PrepHT Cartridge 21.2 x 250 7 877952-101 877952-105 877952-108PrepHT endfittings, 2/pk 820400-901 820400-901 820400-901PrepHT Guard Cartridge, 2/pk 17 x 7.5 5Guard Cartridge Hardware*These columns ship containing reversed phase solvents. Flush with isopropanol before using normal phase solvents. These columns can also be used in HILIC mode.Order online at www.agilent.com/chem/store65
- Page 15 and 16: HPLC Column Selection GuidelinesMet
- Page 17 and 18: HPLC Column Selection GuidelinesOpt
- Page 20 and 21: HPLC Column Selection GuidelinesCar
- Page 22 and 23: Columns for Analytical HPLCPoroshel
- Page 24 and 25: Columns for Analytical HPLCEnvironm
- Page 26 and 27: Columns for Analytical HPLCZORBAX E
- Page 28 and 29: Columns for Analytical HPLCRapid An
- Page 30 and 31: Columns for Analytical HPLCZORBAX E
- Page 32 and 33: Columns for Analytical HPLCZORBAX E
- Page 34 and 35: Columns for Analytical HPLCOOOOOCH
- Page 36 and 37: Columns for Analytical HPLCColumn S
- Page 38 and 39: Columns for Analytical HPLCZORBAX E
- Page 40 and 41: Columns for Analytical HPLCZORBAX E
- Page 42 and 43: Columns for Analytical HPLCZORBAX 8
- Page 44 and 45: Columns for Analytical HPLCSB-CN Op
- Page 46 and 47: Columns for Analytical HPLCZORBAX 8
- Page 48 and 49: Columns for Analytical HPLCZORBAX 8
- Page 50 and 51: Columns for Analytical HPLCZORBAX 8
- Page 52 and 53: Columns for Analytical HPLCRx-C8 an
- Page 54 and 55: Columns for Analytical HPLCZORBAX 8
- Page 56 and 57: Columns for Analytical HPLCExtend-C
- Page 58 and 59: Columns for Analytical HPLCR 1O SiP
- Page 60 and 61: Columns for Analytical HPLCZORBAX B
- Page 62 and 63: Columns for Analytical HPLCZORBAX B
- Page 64 and 65: Columns for Analytical HPLCZORBAX N
- Page 68 and 69: Columns for Analytical HPLCZORBAX H
- Page 70 and 71: Columns for Analytical HPLCZORBAX M
- Page 72 and 73: Columns for Analytical HPLCZORBAX M
- Page 74 and 75: Columns for Analytical HPLCCough-Co
- Page 76 and 77: Columns for Special ApplicationsZOR
- Page 78 and 79: Columns for Special ApplicationsSub
- Page 80 and 81: Columns for Special Applications110
- Page 82 and 83: Columns for Special ApplicationsRed
- Page 84 and 85: Columns for Special ApplicationsRap
- Page 86 and 87: Columns for Special ApplicationsRap
- Page 88 and 89: Columns for Special ApplicationsRun
- Page 90 and 91: Columns for Special ApplicationsZOR
- Page 92 and 93: Columns for Special ApplicationsZOR
- Page 94 and 95: Columns for Special ApplicationsZOR
- Page 96 and 97: Columns for Special ApplicationsZOR
- Page 98 and 99: Columns for Special ApplicationsSca
- Page 100 and 101: Columns for Special ApplicationsZOR
- Page 102 and 103: Columns for Special ApplicationsAgi
- Page 104 and 105: Columns for Special ApplicationsAgi
- Page 106 and 107: Columns for Special ApplicationsSep
- Page 108 and 109: Columns for Bioanalytical Chromatog
- Page 110 and 111: Columns for Bioanalytical Chromatog
- Page 112 and 113: Columns for Bioanalytical Chromatog
- Page 114 and 115: Columns for Bioanalytical Chromatog
<strong>Column</strong>s for Analytical <strong>HPLC</strong>Normal Phase <strong>Column</strong>s Based on ZORBAX Original SILHardware DescriptionSize (mm)Particle Size(µm)SILUSP L3CNUSP L10NH2USP L8CarbohydrateAnalysisStandard <strong>Column</strong>s (no special hardware required)Semi-Prep 9.4 x 250 5 880952-201 880952-205 880952-208Analytical 4.6 x 250 5 880952-701 880952-705 880952-708 840300-908*Analytical 4.6 x 150 5 883952-701 883952-705 883952-708 843300-908*Narrow Bore 2.1 x 50 5 860700-708Guard <strong>Column</strong>s (hardware required)Guard Cartridge, 2/pk 9.4 x 15 5 820675-119 820675-111 820675-111Guard Cartridges, 4/pk 4.6 x 12.5 5 820950-901 820950-905 820950-908 820950-908*Guard Cartridge, 4/pk 2.1 x 12.5 5Guard Hardware Kit 840140-901 840140-901 840140-901Guard Hardware Kit 820888-901 820888-901 820888-901 820888-901*PrepHT Cartridge <strong>Column</strong>s (require endfittings kit 820400-901)PrepHT Cartridge 21.2 x 250 7 877952-101 877952-105 877952-108PrepHT endfittings, 2/pk 820400-901 820400-901 820400-901PrepHT Guard Cartridge, 2/pk 17 x 7.5 5Guard Cartridge Hardware*These columns ship containing reversed phase solvents. Flush with isopropanol before using normal phase solvents. These columns can also be used in HILIC mode.Order online at www.agilent.com/chem/store65