Agilent HPLC Column Selection Guide
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Agilent HPLC Column Selection Guide

Agilent HPLC Column Selection Guide Agilent HPLC Column Selection Guide

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13.07.2015 Views

Columns for Analytical HPLCZORBAX Normal Phase ColumnsFor normal-phase chromatography, the Agilent ZORBAX product line offers a choice ofbonded and non-bonded silica packings.ZORBAX Rx-SIL• Made from highly pure (>99.995%) porous silica microspheres (pore size is the spacebetween the solid silica microparticles)• Available in 1.8 and 5 µm particle sizes• Stronger than other silica types• Less acidic than ZORBAX-SIL, lower metal content• Low acidity and low metal content make ZORBAX Rx-SIL ideal for normal-phase separationof polar compounds that exhibit poor peak symmetry on more acidic silica• Useful for very hydrophilic compounds with high organic mobile phases in HILIC modeZORBAX Eclipse XDB-CN• Made from highly pure Rx-SIL• Excellent choice for normal phase applications with basic compounds• Equilibrates more rapidly than ZORBAX Rx-SIL and is used for many of the samenormal-phase applicationsZORBAX CN• Cyanopropyldimethylsilane monolayer bonded to ZORBAX SIL• Equilibrates more rapidly than ZORBAX SIL, and used for many of the samenormal-phase applications• Less prone to fouling and less water sensitive than silicaZORBAX NH 2• Amino-propyl silane phase bonded to ZORBAX SIL• Used for normal phase and weak anion-exchange, and reversed-phase HPLCof polar compounds• Vitamins A and D are separated in the normal-phase mode• Carbohydrates and sugars are separated in the reversed-phase mode62 Order online at www.agilent.com/chem/store

Columns for Analytical HPLCColumn SpecificationsBonded Phase Pore SizeSurfaceArea pH Range Endcapped Carbon LoadZORBAX Rx-SIL 80Å 180 m 2 /g 0-8.0 NoZORBAX Eclipse XDB-CN 80Å 180 m 2 /g 2.0-8.0 Yes 4.3%ZORBAX SIL 70Å 300 m 2 /g 0-8.0 NoZORBAX CN 70Å 300 m 2 /g 2.0-7.0 Yes 7%ZORBAX NH 2 70Å 300 m 2 /g 2.0-7.0 Yes 4%High Resolution Normal Phase Separationof Octylphenoxy Ethanol Surfactant on ZORBAX CNColumn: ZORBAX CN880952-7054.6 x 250 mm, 5 µmMobile Phase: Primary: HeptaneSecondary: 2-Methoxyethanol/Isopropanol (50/50)Flow Rate: 2 mL/minGradient: 2-20% Secondary in 10 min., Linear Hold at 20%Temperature: 50°CDetector: 278 nmSample:Octylphenoxy (polyethylene oxy)Ethanol Surfactant (n= 10)0 510 15Time (min)20LCNP001Order online at www.agilent.com/chem/store63

<strong>Column</strong>s for Analytical <strong>HPLC</strong><strong>Column</strong> SpecificationsBonded Phase Pore SizeSurfaceArea pH Range Endcapped Carbon LoadZORBAX Rx-SIL 80Å 180 m 2 /g 0-8.0 NoZORBAX Eclipse XDB-CN 80Å 180 m 2 /g 2.0-8.0 Yes 4.3%ZORBAX SIL 70Å 300 m 2 /g 0-8.0 NoZORBAX CN 70Å 300 m 2 /g 2.0-7.0 Yes 7%ZORBAX NH 2 70Å 300 m 2 /g 2.0-7.0 Yes 4%High Resolution Normal Phase Separationof Octylphenoxy Ethanol Surfactant on ZORBAX CN<strong>Column</strong>: ZORBAX CN880952-7054.6 x 250 mm, 5 µmMobile Phase: Primary: HeptaneSecondary: 2-Methoxyethanol/Isopropanol (50/50)Flow Rate: 2 mL/minGradient: 2-20% Secondary in 10 min., Linear Hold at 20%Temperature: 50°CDetector: 278 nmSample:Octylphenoxy (polyethylene oxy)Ethanol Surfactant (n= 10)0 510 15Time (min)20LCNP001Order online at www.agilent.com/chem/store63

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