13.07.2015 Views

Agilent HPLC Column Selection Guide

Agilent HPLC Column Selection Guide

Agilent HPLC Column Selection Guide

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<strong>Column</strong>s for Analytical <strong>HPLC</strong>ZORBAX Normal Phase <strong>Column</strong>sFor normal-phase chromatography, the <strong>Agilent</strong> ZORBAX product line offers a choice ofbonded and non-bonded silica packings.ZORBAX Rx-SIL• Made from highly pure (>99.995%) porous silica microspheres (pore size is the spacebetween the solid silica microparticles)• Available in 1.8 and 5 µm particle sizes• Stronger than other silica types• Less acidic than ZORBAX-SIL, lower metal content• Low acidity and low metal content make ZORBAX Rx-SIL ideal for normal-phase separationof polar compounds that exhibit poor peak symmetry on more acidic silica• Useful for very hydrophilic compounds with high organic mobile phases in HILIC modeZORBAX Eclipse XDB-CN• Made from highly pure Rx-SIL• Excellent choice for normal phase applications with basic compounds• Equilibrates more rapidly than ZORBAX Rx-SIL and is used for many of the samenormal-phase applicationsZORBAX CN• Cyanopropyldimethylsilane monolayer bonded to ZORBAX SIL• Equilibrates more rapidly than ZORBAX SIL, and used for many of the samenormal-phase applications• Less prone to fouling and less water sensitive than silicaZORBAX NH 2• Amino-propyl silane phase bonded to ZORBAX SIL• Used for normal phase and weak anion-exchange, and reversed-phase <strong>HPLC</strong>of polar compounds• Vitamins A and D are separated in the normal-phase mode• Carbohydrates and sugars are separated in the reversed-phase mode62 Order online at www.agilent.com/chem/store

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