13.07.2015 Views

Agilent HPLC Column Selection Guide

Agilent HPLC Column Selection Guide

Agilent HPLC Column Selection Guide

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<strong>Column</strong>s for Analytical <strong>HPLC</strong>ZORBAX Bonus-RP Provides Unique Selectivity<strong>Column</strong> A: ZORBAX Bonus-RP A883668-9014.6 x 150 mm, 5 µmmAu<strong>Column</strong> B: Eclipse XDB-C815993967-9064.6 x 150 mm, 5 µm10Mobile Phase: 75% 25 mM Na Citrate, pH 625% MeOH5Flow Rate: 1.0 mL/min0Temperature: AmbientDetector: 254 nmSample: 3 µLBCephalosporinsmAu1. Cephalexin2. Cephaclor3. Cephuroxime4. Cephoxitin0 213244 6 8 10Time (min)1Peak elution order can change dramatically when usingBonus-RP. In this example, the elution order of the firstthree peaks change.1510532400 24 6 8 10Time (min)LCBR005ZORBAX Bonus-RPHardware DescriptionSize (mm)Particle Size(µm)Bonus-RPUSP L60Standard <strong>Column</strong>s (no special hardware required)Analytical 4.6 x 250 5 880668-901Analytical 4.6 x 150 5 883668-901Rapid Resolution 4.6 x 250 3.5 884950-577Rapid Resolution 4.6 x 150 3.5 863668-901Rapid Resolution 4.6 x 100 3.5 864668-901Rapid Resolution 4.6 x 75 3.5 866668-901Rapid Resolution 4.6 x 50 3.5 835668-901Order online at www.agilent.com/chem/store59

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