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<strong>Column</strong>s for Analytical <strong>HPLC</strong>ZORBAX Bonus-RP is Stable at Low and Mid pH<strong>Column</strong>: ZORBAX Bonus-RP883668-9014.6 x 150 mm, 5 µmMobile Phase: 60% 25 mMPhosphate Buffer,pH 7.0: 40% ACNFlow Rate: 1.5 mL/minTemperature: 23°CToluene Plate Height, cm0.00180.00160.0014Competitive alkyl/amideBonus-RPTriple endcapping of Bonus-RP enhances stabilityat pH 7. Each 10,000 column volumes is equivalentto approximately one working month.0.00120.00100 5,000 10,000 15,000 20,000 25,000 30,000 35,000<strong>Column</strong> Volume of PurgeLCBR003Dimethyl-C18/amide, Bonus-RP<strong>Column</strong>:ZORBAX Bonus-RP883668-9014.6 x 150 mm, 5 µmMobile Phase:Flow Rate:Temperature:Aging:50% MeOH50% 0.1% TFATest:80% MeOH20% H2O1.0 mL/minAging:60°CTest:23°CToluene Plate Height, cm0.00550.00500.00450.00400.00350.0030Dimethyl-C18/amideBonus-RPSterically protecting side groups provide goodlow pH stability and longer column lifetime thansimilar polar alkyl bonded phases.0.00250.00200 10,000 20,000 30,000 40,000<strong>Column</strong> Volume of PurgeLCBR00458 Order online at www.agilent.com/chem/store
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