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<strong>Column</strong>s for Analytical <strong>HPLC</strong>Improved Peak Shape of Basic Compounds Using Bonus-RP<strong>Column</strong>: Alkyl-C84.6 x 150 mm, 5 µmMobile Phase: 75% 25 mM NH 4 OAc, pH 5.525% ACNFlow Rate: 1.5 mL/minTemperature: 40°CDetector: 254 nmBonus-RP eliminates peak tailing of these basic compounds in comparison to atypical alkyl C8 bonded phase. In the mid-pH region, residual silanols caninteract more strongly with basic compounds to cause peak tailing. The polargroup in the Bonus-RP bonded phase eliminates peak tailing of these basiccompounds by reducing interactions with residual silanols.TF1. 1.82. 1.73. 2.1121. Doxylamine2. Chlorpheniramine3. Triprolidine30 1 2 3 4 5 6 7 8 9Time (min)10LCBR001<strong>Column</strong>: ZORBAX Bonus-RP883668-9014.6 x 150 mm, 5 µmMobile Phase: 80% 25 mM NH 4 OAc, pH 5.520% ACNFlow Rate: 1.5 mL/minTemperature: 40°CDetector: 254 nmTF1. 1.12. 1.13. 1.11231. Doxylamine2. Chlorpheniramine3. Triprolidine0 1 2 3 4 5 6Time (min)LCBR002Order online at www.agilent.com/chem/store57