13.07.2015 Views

Agilent HPLC Column Selection Guide

Agilent HPLC Column Selection Guide

Agilent HPLC Column Selection Guide

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<strong>Column</strong>s for Analytical <strong>HPLC</strong>R 1O SiPGR 1CH 3Si CH 3R 1O SiPGR 1CH 3Si CH 3CH 3CH 3R 1O SiPGR 1Unique, Polar Alkyl Bonus-RP Bonded PhaseRRRZORBAX Bonus-RP• Excellent peak shape for challenging basic compounds at low and mid pH• Unique reversed-phase selectivity• Novel bonding technology with embedded polar group and steric protection• Usable in 100% aqueous mobile phasesThe <strong>Agilent</strong> ZORBAX Bonus-RP column has a polar amide group embedded in a long alkylchain. This novel bonding reduces interactions between basic compounds and the silicasupport, improving peak shape for the most difficult basic compounds. Peak shape andcolumn lifetime are further improved by triple endcapping. In addition, diisopropyl side groupsprovide steric protection against acid hydrolysis for good lifetime at low pH. The Bonus-RPcolumn provides an alternate selectivity to C18 and C8 alkyl bonded phases.<strong>Column</strong> SpecificationsBonded PhasePoreSizeSurfaceAreaTemp.Limits* pH Range EndcappedCarbonLoadZORBAX Bonus-RP 80Å 180 m 2 /g 60°C 2.0-9.0 Triple 9.5%Specifications represent typical values only.*Temperature limits are 60°C up to pH 8, 40°C from pH 8-9.56 Order online at www.agilent.com/chem/store

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