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<strong>Column</strong>s for Analytical <strong>HPLC</strong>Extend-C18 Provides Good Peak Shape at Low pH<strong>Column</strong>: ZORBAX Extend-C18773450-9024.6 x 150 mm, 5 µmMobile Phase: 80% 25 mM NaH 2 PO 4 , pH 3.020% MethanolFlow Rate: 1.0 mL/minTemperature: 35°CDetector: 254 nmSample:Basic Compounds1231. Theobromine2. Dimethylxanthine3. Theophylline4. Caffeine4These basic compounds are separated on the Extend-C18at low pH with excellent peak shape. The Extend-C18column can be used at high and low pH.0 12 34 5 67Time (min)LC80003ZORBAX 80Å Extend-C18Hardware DescriptionSize (mm)Particle Size(µm)Extend-C18USP L1Standard <strong>Column</strong>s (no special hardware required)Analytical 4.6 x 250 5 770450-902Analytical 4.6 x 150 5 773450-902Analytical 4.6 x 50 5 746450-902Rapid Resolution 4.6 x 150 3.5 763953-902Rapid Resolution 4.6 x 100 3.5 764953-902Rapid Resolution 4.6 x 75 3.5 766953-902Rapid Resolution 4.6 x 50 3.5 735953-90254 Order online at www.agilent.com/chem/store
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