Agilent HPLC Column Selection Guide
Agilent HPLC Column Selection Guide Agilent HPLC Column Selection Guide
Columns for Analytical HPLCRx-C8 and SB-C8 are the Same ColumnColumn A: ZORBAX Rx-C8880967-9014.6 x 250 mm, 5 µmColumn B: ZORBAX SB-C8880975-9064.6 x 250 mm, 5 µmSample: Sulfa Drugs0.125 mg/mL each in waterFlow Rate: 1.5 mL/minGradient: 10% B at t n , increase to 35% Bover 25 min, decrease to 10% Bin 5 min, hold 10% B for 10 minTCC: 26°CDAD:254 nmA12345671. Sulfadiazine2. Sulfapyridine3. Sulfamerazine4. Sulfamethazine5. Sulfamethoxypyridazine6. Sulfachloropyridazine7. Sulfamethoxazole8. Sulfadimethoxine8B52310 15 20 25Time (min)1456875 10 15 20 25Time (min)RxC8_SBC850 Order online at www.agilent.com/chem/store
Columns for Analytical HPLCZORBAX RxHardware DescriptionParticle SizeSize (mm) (µm)Rx-C18USP L1Rx-C8USP L7**Semi-Preparative 9.4 x 250 5 880967-202 880967-201Analytical 4.6 x 250 5 880967-902 880967-901Analytical 4.6 x 150 5 883967-902 883967-901Rapid Resolution 4.6 x 150 3.5 863967-902 863953-906Rapid Resolution 4.6 x 100 3.5 861967-902 861953-906Rapid Resolution 4.6 x 75 3.5 866967-902 866953-906Solvent Saver 3.0 x 250 5 880967-302 880975-306Solvent Saver 3.0 x 150 5 883967-302 883975-306Solvent Saver Plus 3.0 x 150 3.5 863967-302 863954-306Solvent Saver Plus 3.0 x 100 3.5 861967-302 861954-306Narrow Bore 2.1 x 150 5 883700-902 883700-906Narrow Bore RR* 2.1 x 100 3.5 861767-902 861753-906Guard Cartridge, 2/pk 9.4 x 15 7 820675-115 820675-115Guard Cartridge, 4/pk 4.6 x 12.5 5 820950-914 820950-913Guard Cartridge, 4/pk 2.1 x 12.5 5 821125-915 821125-915Guard Hardware Kit 9.4 x 15 840140-901 840140-901Guard Hardware Kit 820888-901 820888-901PrepHT Cartridge Columns (require endfittings kit 820400-901)PrepHT Cartridge 21.2 x 250 7 877967-102 877250-106PrepHT Cartridge 21.2 x 150 7 877150-106PrepHT Cartridge 21.2 x 150 5 870150-906PrepHT Cartridge 21.2 x 100 5 870100-906PrepHT Cartridge 21.2 x 50 5 870050-906PrepHT Guard Cartridge, 2/pk 820212-914 820212-915Guard Cartridge Hardware 820444-901 820444-901PrepHT endfittings, 2/pk 820400-901 820400-901*RR: Rapid Resolution 3.5 µm**Rx-C8 is the same product as SB-C8Order online at www.agilent.com/chem/store51
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<strong>Column</strong>s for Analytical <strong>HPLC</strong>ZORBAX RxHardware DescriptionParticle SizeSize (mm) (µm)Rx-C18USP L1Rx-C8USP L7**Semi-Preparative 9.4 x 250 5 880967-202 880967-201Analytical 4.6 x 250 5 880967-902 880967-901Analytical 4.6 x 150 5 883967-902 883967-901Rapid Resolution 4.6 x 150 3.5 863967-902 863953-906Rapid Resolution 4.6 x 100 3.5 861967-902 861953-906Rapid Resolution 4.6 x 75 3.5 866967-902 866953-906Solvent Saver 3.0 x 250 5 880967-302 880975-306Solvent Saver 3.0 x 150 5 883967-302 883975-306Solvent Saver Plus 3.0 x 150 3.5 863967-302 863954-306Solvent Saver Plus 3.0 x 100 3.5 861967-302 861954-306Narrow Bore 2.1 x 150 5 883700-902 883700-906Narrow Bore RR* 2.1 x 100 3.5 861767-902 861753-906Guard Cartridge, 2/pk 9.4 x 15 7 820675-115 820675-115Guard Cartridge, 4/pk 4.6 x 12.5 5 820950-914 820950-913Guard Cartridge, 4/pk 2.1 x 12.5 5 821125-915 821125-915Guard Hardware Kit 9.4 x 15 840140-901 840140-901Guard Hardware Kit 820888-901 820888-901PrepHT Cartridge <strong>Column</strong>s (require endfittings kit 820400-901)PrepHT Cartridge 21.2 x 250 7 877967-102 877250-106PrepHT Cartridge 21.2 x 150 7 877150-106PrepHT Cartridge 21.2 x 150 5 870150-906PrepHT Cartridge 21.2 x 100 5 870100-906PrepHT Cartridge 21.2 x 50 5 870050-906PrepHT Guard Cartridge, 2/pk 820212-914 820212-915Guard Cartridge Hardware 820444-901 820444-901PrepHT endfittings, 2/pk 820400-901 820400-901*RR: Rapid Resolution 3.5 µm**Rx-C8 is the same product as SB-C8Order online at www.agilent.com/chem/store51
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