13.07.2015 Views

Agilent HPLC Column Selection Guide

Agilent HPLC Column Selection Guide

Agilent HPLC Column Selection Guide

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<strong>Column</strong>s for Analytical <strong>HPLC</strong>Rx-C8 and SB-C8 are the Same <strong>Column</strong><strong>Column</strong> A: ZORBAX Rx-C8880967-9014.6 x 250 mm, 5 µm<strong>Column</strong> B: ZORBAX SB-C8880975-9064.6 x 250 mm, 5 µmSample: Sulfa Drugs0.125 mg/mL each in waterFlow Rate: 1.5 mL/minGradient: 10% B at t n , increase to 35% Bover 25 min, decrease to 10% Bin 5 min, hold 10% B for 10 minTCC: 26°CDAD:254 nmA12345671. Sulfadiazine2. Sulfapyridine3. Sulfamerazine4. Sulfamethazine5. Sulfamethoxypyridazine6. Sulfachloropyridazine7. Sulfamethoxazole8. Sulfadimethoxine8B52310 15 20 25Time (min)1456875 10 15 20 25Time (min)RxC8_SBC850 Order online at www.agilent.com/chem/store

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