Agilent HPLC Column Selection Guide
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Agilent HPLC Column Selection Guide

Agilent HPLC Column Selection Guide Agilent HPLC Column Selection Guide

team.cag.com
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13.07.2015 Views

Columns for Analytical HPLCZORBAX 80Å StableBondDescription Size (mm) Particle Size (µm)SB-C18USP L1Capillary Glass-lined ColumnsCapillary 0.5 x 250 5 5064-8258Capillary 0.5 x 150 5 5064-8256Capillary 0.5 x 35 5 5064-8254Capillary RR 0.5 x 150 3.5 5064-8262Capillary RR 0.5 x 35 3.5 5064-8260Capillary 0.3 x 250 5 5064-8257Capillary 0.3 x 150 5 5064-8255Capillary 0.3 x 35 5 5064-8253Capillary RR 0.3 x 150 3.5 5064-826148 Order online at www.agilent.com/chem/store

Columns for Analytical HPLCZORBAX Rx• Rx-C18 is recommended for alternate selectivity at low pH relative to Eclipse XDB-C18and StableBond SB-C18; for higher temperature applications, StableBond is recommended.This column has a higher carbon load than SB-C18 columns (12% vs. 10%).• Rx-C18 offers high stability and good peak shape for low pH applications.• Rx-C18 is manufactured using dimethyloctadecylsilane, is non-endcapped and providesexcellent stability up to pH 8.• Rx-C8 is the same product as SB-C8.Column SpecificationsBonded PhasePoreSizeSurfaceAreaTemp.LimitspHRange*EndcappedCarbonLoadZORBAX Rx-C18 80Å 180 m 2 /g 60°C 2.0-8.0 No 12%ZORBAX Rx-C8 80Å 180 m 2 /g 80°C 1.0-8.0 No 5.5%Specifications represent typical values only.*At pH 6-9 highest column stability for all silica based columns is obtained by operating at temperatures < 40°C and usinglower buffer concentrations in the range of 0.01 - 0.02M.Analysis of Diazepam on Rx-C18Column: ZORBAX Rx-C18880967-3023.0 x 250 mm, 5 µmMobile Phase: 35% H 2 O: 65% MeOHFlow Rate: 0.5 mL/min121. Ethylparaben2. DiazepamAn Rx-C18 column is used for this USP analysis of diazepam and the internal standardethylparaben. The Solvent Saver 3.0 mm i.d. Rx-C18 column reduces solvent usage by60% over what would be used if the analysis were done on a 4.6 x 250 mm column.0.0 2.5 5.0 7.5Time (min)LCRX001Order online at www.agilent.com/chem/store49

<strong>Column</strong>s for Analytical <strong>HPLC</strong>ZORBAX Rx• Rx-C18 is recommended for alternate selectivity at low pH relative to Eclipse XDB-C18and StableBond SB-C18; for higher temperature applications, StableBond is recommended.This column has a higher carbon load than SB-C18 columns (12% vs. 10%).• Rx-C18 offers high stability and good peak shape for low pH applications.• Rx-C18 is manufactured using dimethyloctadecylsilane, is non-endcapped and providesexcellent stability up to pH 8.• Rx-C8 is the same product as SB-C8.<strong>Column</strong> SpecificationsBonded PhasePoreSizeSurfaceAreaTemp.LimitspHRange*EndcappedCarbonLoadZORBAX Rx-C18 80Å 180 m 2 /g 60°C 2.0-8.0 No 12%ZORBAX Rx-C8 80Å 180 m 2 /g 80°C 1.0-8.0 No 5.5%Specifications represent typical values only.*At pH 6-9 highest column stability for all silica based columns is obtained by operating at temperatures < 40°C and usinglower buffer concentrations in the range of 0.01 - 0.02M.Analysis of Diazepam on Rx-C18<strong>Column</strong>: ZORBAX Rx-C18880967-3023.0 x 250 mm, 5 µmMobile Phase: 35% H 2 O: 65% MeOHFlow Rate: 0.5 mL/min121. Ethylparaben2. DiazepamAn Rx-C18 column is used for this USP analysis of diazepam and the internal standardethylparaben. The Solvent Saver 3.0 mm i.d. Rx-C18 column reduces solvent usage by60% over what would be used if the analysis were done on a 4.6 x 250 mm column.0.0 2.5 5.0 7.5Time (min)LCRX001Order online at www.agilent.com/chem/store49

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