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<strong>HPLC</strong> <strong>Column</strong> <strong>Selection</strong> <strong>Guide</strong>lines<strong>HPLC</strong> column selectionand method development strategiesTo make it easier to select the right column for your specific application, we have includedthe following reference material so you can maximize your most valuable resource – time.<strong>HPLC</strong> <strong>Column</strong> <strong>Selection</strong> Flow ChartHere, you will find step-by-step instructions on choosing an initial column for methoddevelopment, based on key separation considerations, such as analyte and mobile phase.We will then point you to the page that has the exact column you need.Simple <strong>Guide</strong>lines for Choosing a Reversed Phase <strong>HPLC</strong> <strong>Column</strong>We will show you how to select the best column for small molecule and protein/peptideanalysis, based on factors such as bonded phase and column configuration.Quick <strong>Guide</strong> to USP Categories For <strong>HPLC</strong> <strong>Column</strong>sThis section clearly and concisely lists the U.S. Pharmacopeia categories for <strong>Agilent</strong>’s ZORBAXcolumn family, so you can easily select the columns that conform to USP or otherpharmacopoeial monographs.Reversed-Phase <strong>HPLC</strong> Method DevelopmentChromatographic resolution between two or more peaks depends upon column efficiency,selectivity, and retention – all factors that are affected by pH. While one type of column maydo many separations, this section describes a complete method development strategy basedon changing the pH of the mobile phase.Order online at www.agilent.com/chem/store3
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