Agilent HPLC Column Selection Guide
Share Embed Flag
Download ePaper

Agilent HPLC Column Selection Guide

Agilent HPLC Column Selection Guide Agilent HPLC Column Selection Guide

team.cag.com
from team.cag.com More from this publisher
13.07.2015 Views

Columns for Analytical HPLCSB-CN Optimizes Retention and ResolutionColumn A: ZORBAX SB-C18866953-9024.6 x 75 mm, 3.5 µmColumn B: ZORBAX SB-CN866953-9054.6 x 75 mm, 3.5 µmMobile Phase: 30% ACN70% 25mM NaH 2 PO 4 , pH 2.5Flow Rate: 1.0 mL/minTemperature: 35°CA12341. Estriol2. Daidzen3. Quercetin4. Genistein5. Diethylstilbestrol5B21 3450 5 10 15 20 25Time (min)0 2 4 6 8 10 12Time (min)The SB-CN column is used here to reduce analysis time by 50%. The retention of the most hydrophobic analyte is cut in half. At the same time retention of the more polar,early eluting peaks increases slightly.LCSB003More RRHT Bonded Phase ChoicesAllow for Rapid Method Development OptimizationColumn A: ZORBAX SB-C18827975-9024.6 x 50 mm, 1.8 µmColumn B: SB-C8827975-9064.6 x 50 mm, 1.8 µmColumn C: ZORBAX SB-Phenyl827975-9124.6 x 50 mm, 1.8 µmColumn D: ZORBAX SB-CN827975-9054.6 x 50 mm, 1.8 µmMobile Phase: Acetonitrile (40%):Buffer 60% pH=2.425mM NaH 2 PO 4Flow Rate: 1.85 mL/minDetector: DAD, 254 nmLC: Agilent 1200 RR LCSample:As listed, 1 µL injection5 Different Bonded Phase Choices in StableBondfamily enhance method optimization.Note: SB-Aq not shown.mAU4504003503002502001501005000ABCD11. Tolmetin2. Naproxen3. Diflunisal4. Ibuprofen5. DiclofenacChange in Elution Order Peaks 3, 4 & 521351 2 3541325425341 2 3 4 54minMoreRRHT42 Order online at www.agilent.com/chem/store

Columns for Analytical HPLCFive Different Bonded Phases Provide Selectivity OptionsColumn A:Column B:ZORBAX SB-C18883975-9024.6 x 150 mm, 5 µmZORBAX SB-C8883975-9064.6 x 150 mm, 5 µmA13254Column C:Column D:Column E:Mobile Phase:ZORBAX SB-C3883975-9094.6 x 150 mm, 5 µmZORBAX SB-Phenyl883975-9124.6 x 150 mm, 5 µmZORBAX SB-CN883975-9054.6 x 150 mm, 5 µm0-100% B in 18.8 minA: 50 mM NaH 2 PO 4 ,pH 2.5 in 95% H 2 O / 5% ACNB: 50 mM NaH 2 PO 4 ,pH 2.5 in 47% H 2 O / 53% ACNBC113235454Flow Rate: 1.0 mL/minTemperature: 26°CDetector: 254 nmSample:1. Procaine2. Lidocaine3. d-Cinchonine4. Butacaine5. TetracaineD231452SB-C3 is just one of the five different StableBond selectivity choices. In thisexample, optimum resolution is obtained with SB-C3. All are based on the samehigh purity Rx-SIL. Selectivity changes are therefore dependent only on the bondedphases, making method development more reliable.E132450 5 1015Time (min)LCSB004Order online at www.agilent.com/chem/store43

<strong>Column</strong>s for Analytical <strong>HPLC</strong>Five Different Bonded Phases Provide Selectivity Options<strong>Column</strong> A:<strong>Column</strong> B:ZORBAX SB-C18883975-9024.6 x 150 mm, 5 µmZORBAX SB-C8883975-9064.6 x 150 mm, 5 µmA13254<strong>Column</strong> C:<strong>Column</strong> D:<strong>Column</strong> E:Mobile Phase:ZORBAX SB-C3883975-9094.6 x 150 mm, 5 µmZORBAX SB-Phenyl883975-9124.6 x 150 mm, 5 µmZORBAX SB-CN883975-9054.6 x 150 mm, 5 µm0-100% B in 18.8 minA: 50 mM NaH 2 PO 4 ,pH 2.5 in 95% H 2 O / 5% ACNB: 50 mM NaH 2 PO 4 ,pH 2.5 in 47% H 2 O / 53% ACNBC113235454Flow Rate: 1.0 mL/minTemperature: 26°CDetector: 254 nmSample:1. Procaine2. Lidocaine3. d-Cinchonine4. Butacaine5. TetracaineD231452SB-C3 is just one of the five different StableBond selectivity choices. In thisexample, optimum resolution is obtained with SB-C3. All are based on the samehigh purity Rx-SIL. Selectivity changes are therefore dependent only on the bondedphases, making method development more reliable.E132450 5 1015Time (min)LCSB004Order online at www.agilent.com/chem/store43

logo

products

Resources

Company

Terms of service
Privacy policy
Cookie policy
Cookie settings
Imprint
Change language
Made with love in Switzerland
© 2024 Yumpu.com all rights reserved

Hooray! Your file is uploaded and ready to be published.

Saved successfully!

Ooh no, something went wrong!