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<strong>Column</strong>s for Analytical <strong>HPLC</strong>SB-CN Optimizes Retention and Resolution<strong>Column</strong> A: ZORBAX SB-C18866953-9024.6 x 75 mm, 3.5 µm<strong>Column</strong> B: ZORBAX SB-CN866953-9054.6 x 75 mm, 3.5 µmMobile Phase: 30% ACN70% 25mM NaH 2 PO 4 , pH 2.5Flow Rate: 1.0 mL/minTemperature: 35°CA12341. Estriol2. Daidzen3. Quercetin4. Genistein5. Diethylstilbestrol5B21 3450 5 10 15 20 25Time (min)0 2 4 6 8 10 12Time (min)The SB-CN column is used here to reduce analysis time by 50%. The retention of the most hydrophobic analyte is cut in half. At the same time retention of the more polar,early eluting peaks increases slightly.LCSB003More RRHT Bonded Phase ChoicesAllow for Rapid Method Development Optimization<strong>Column</strong> A: ZORBAX SB-C18827975-9024.6 x 50 mm, 1.8 µm<strong>Column</strong> B: SB-C8827975-9064.6 x 50 mm, 1.8 µm<strong>Column</strong> C: ZORBAX SB-Phenyl827975-9124.6 x 50 mm, 1.8 µm<strong>Column</strong> D: ZORBAX SB-CN827975-9054.6 x 50 mm, 1.8 µmMobile Phase: Acetonitrile (40%):Buffer 60% pH=2.425mM NaH 2 PO 4Flow Rate: 1.85 mL/minDetector: DAD, 254 nmLC: <strong>Agilent</strong> 1200 RR LCSample:As listed, 1 µL injection5 Different Bonded Phase Choices in StableBondfamily enhance method optimization.Note: SB-Aq not shown.mAU4504003503002502001501005000ABCD11. Tolmetin2. Naproxen3. Diflunisal4. Ibuprofen5. DiclofenacChange in Elution Order Peaks 3, 4 & 521351 2 3541325425341 2 3 4 54minMoreRRHT42 Order online at www.agilent.com/chem/store
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