Agilent HPLC Column Selection Guide
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Agilent HPLC Column Selection Guide

Agilent HPLC Column Selection Guide Agilent HPLC Column Selection Guide

team.cag.com
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13.07.2015 Views

Columns for Analytical HPLCZORBAX 80Å StableBondROOHSiRRR 1• Longest column lifetime and best reproducibility for low pH separations – down to pH 1• Patented stable column chemistry allows use at high temperature and low pHwithout degradation• Six different bonded phases provide broad selectivity – SB-C18, SB-C8, SB-CN, SB-Phenyl,SB-C3, SB-Aq• High purity (Type B) silica for good peak shapeOOHOSiRRSiRR 1R 1Agilent ZORBAX StableBond columns use patented, unique, nonfunctional silanes with bulkydiisobutyl (SB-C18) or diisopropyl (SB-C8, SB-C3, SB-Phenyl, SB-CN, and SB-Aq) side chaingroups that sterically protect the key siloxane bond to the silica surface from hydrolytic attackat low pH. StableBond packing materials are not endcapped in order to provide exceptionalstability and to maximize lifetime and reproducibility under acidic mobile phase conditions.The high purity, low acidity silica provides excellent peak shape with acidic, basic and neutralcompounds so that StableBond columns are an excellent choice for low pH methoddevelopment. ZORBAX StableBond columns are compatible with all common mobile phases,including very high aqueous mobile phases.Sterically Protected StableBond Bonded PhaseColumn SpecificationsBonded PhasePoreSizeSurfaceAreaTemp.Limits*pH Range* EndcappedCarbonLoadZORBAX SB-C18 80Å 180 m 2 /g 90°C 0.8-8.0 No 10%ZORBAX SB-C8 80Å 180 m 2 /g 80°C 1.0-8.0 No 5.5%ZORBAX SB-C3 80Å 180 m 2 /g 80°C 1.0-8.0 No 4%ZORBAX SB-Phenyl 80Å 180 m 2 /g 80°C 1.0-8.0 No 5.5%ZORBAX SB-CN 80Å 180 m 2 /g 80°C 1.0-8.0 No 4%ZORBAX SB-Aq 80Å 180 m 2 /g 80°C 1.0-8.0 No proprietarySpecifications represent typical values only.*StableBond columns are designed for optimal use at low pH. At pH 6-8, highest column stability for all silica-basedcolumns is obtained by operating at temperatures

Columns for Analytical HPLCStableBond SB-C18 Shows Excellent Stability at Low pH and High Temperature (pH 0.8, 90°C)Column:Column:ZORBAX SB-C18883975-9024.6 x 150 mm, 5 µmZORBAX Rx-C18883967-9024.6 x 150 mm, 5 µmMobile Phase: 50% Methanol/50% Waterwith 1.0% TFATest Solute: TolueneTemperature: 90°CAs an indicator of column breakdown, retention time of toluene was measured after purging the column with mobile phase. Only the StableBond SB-C18 is unchangedafter three working months of use under these very low pH (0.8) and high temperature (90°C) conditions. ZORBAX Rx-C18 also provides a stable matrix, and can beused as an alternative selectivity to StableBond SB-C18.StableBond SB-C18 (Diisobutyl-C18)1.00.8ZORBAX Rx-C18 (Dimethyl-C18)Competitor A-C18Relative Retention0.60.4Competitor B-C18Competitor C-C18Competitor D-C180.2Competitor E-C180.00 5,000 10,000 15,000 20,000 25,000 30,000Column Volumes of Mobile Phase PurgeLCSB001Shorter Chain ZORBAX SB-CN is also Stableat Low pH (pH 2.0, 50°C)100SB-CNColumn:ZORBAX SB-CN883975-9054.6 x 150 mm, 5 µm80Mobile Phase: 0.1% TFA, pH 2: ACNFlow Rate: 1 mL/minGradient: 0-100% ACNTemperature: 50°CSample:1-phenylheptane @ 50% AC/50% Water with 0.1% TFA% k Remaining6040Si-(Me) 2 PrCN20ZORBAX StableBond SB-CN and the other short chainStableBond bonded phases are also exceptionally stableat low pH. Conventional dimethyl CN and similar bondedphases lack this stability.00 1,000 2,000 3,000 4,000 5,000 6,000Column VolumesLCSB002Order online at www.agilent.com/chem/store41

<strong>Column</strong>s for Analytical <strong>HPLC</strong>StableBond SB-C18 Shows Excellent Stability at Low pH and High Temperature (pH 0.8, 90°C)<strong>Column</strong>:<strong>Column</strong>:ZORBAX SB-C18883975-9024.6 x 150 mm, 5 µmZORBAX Rx-C18883967-9024.6 x 150 mm, 5 µmMobile Phase: 50% Methanol/50% Waterwith 1.0% TFATest Solute: TolueneTemperature: 90°CAs an indicator of column breakdown, retention time of toluene was measured after purging the column with mobile phase. Only the StableBond SB-C18 is unchangedafter three working months of use under these very low pH (0.8) and high temperature (90°C) conditions. ZORBAX Rx-C18 also provides a stable matrix, and can beused as an alternative selectivity to StableBond SB-C18.StableBond SB-C18 (Diisobutyl-C18)1.00.8ZORBAX Rx-C18 (Dimethyl-C18)Competitor A-C18Relative Retention0.60.4Competitor B-C18Competitor C-C18Competitor D-C180.2Competitor E-C180.00 5,000 10,000 15,000 20,000 25,000 30,000<strong>Column</strong> Volumes of Mobile Phase PurgeLCSB001Shorter Chain ZORBAX SB-CN is also Stableat Low pH (pH 2.0, 50°C)100SB-CN<strong>Column</strong>:ZORBAX SB-CN883975-9054.6 x 150 mm, 5 µm80Mobile Phase: 0.1% TFA, pH 2: ACNFlow Rate: 1 mL/minGradient: 0-100% ACNTemperature: 50°CSample:1-phenylheptane @ 50% AC/50% Water with 0.1% TFA% k Remaining6040Si-(Me) 2 PrCN20ZORBAX StableBond SB-CN and the other short chainStableBond bonded phases are also exceptionally stableat low pH. Conventional dimethyl CN and similar bondedphases lack this stability.00 1,000 2,000 3,000 4,000 5,000 6,000<strong>Column</strong> VolumesLCSB002Order online at www.agilent.com/chem/store41

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