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<strong>Column</strong>s for Analytical <strong>HPLC</strong>Good Peak Shape Over a Wide pH Rangewith ZORBAX Eclipse XDB<strong>Column</strong>: Eclipse XDB-C8993967-9064.6 x 150 mm, 5 µmMobile Phase: A:pH 3.0 75% 25 mMphosphate bufferpH 3.0: 25% ACNB:pH 7.0 90% 20 mMphosphate pH 7.010% ACNFlow Rate: 1.5 mL/minTemperature: 40°CA12345BSample: A:1. Maleate2. Doxylamine3. Chlorpheniramine4. Triprolidine5. Diphenhydramine2 B:1. Procainamide2. N-acetylprocainamide13. N-propionylprocainamide30 2.5 5.0 7.5Time (min)0 2.5 5.0 7.5Time (min)LCEC004ZORBAX Eclipse XDB columns provide good peak shape over a wide pH range and are anexcellent choice for method development from pH 2-9.<strong>Column</strong> Stability Testing at pH 7.0<strong>Column</strong> A: Competitive C8SIL-typeAfter 1826<strong>Column</strong> Volumes<strong>Column</strong> B: Eclipse XDB-C8993967-9064.6 x 150 mm, 5 µmSol-typeAfter 1843<strong>Column</strong> VolumesMobile Phase: 60% ACN40% 250 mM PhosphateBuffer, pH 7.0Flow Rate: 1.5 mL/minTemperature: 60°CSample:Tricyclic AntidepressantsA1B5 1. Uracil152. Nortriptyline2, 33. Doxepin4. Amitriptyline45. Trimipramine2 340 1 2 3 4 5 6 7 8 9 10 11 12Time (min)0 1 2 3 4 5 6 7 8Time (min)LCEC006Double endcapping, dense bonding and the durable Rx-Sil particles (sol-type) combine to provide long lifetime at pH 7 when compared to single endcapped sil-gel columnsused here. The conditions used for this test – high temperature (60°C) and high salt concentration (250 mM), accelerate the dissolution of silica, causing premature failureof the sil-gel type column.Order online at www.agilent.com/chem/store33
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