Agilent HPLC Column Selection Guide
Agilent HPLC Column Selection Guide Agilent HPLC Column Selection Guide
Columns for Analytical HPLCOOOOOCH 3SiCH 3CH 3Si CH 3CH 3CH 3SiCH 3CH 3Si CH 3CH 3CH 3SiCH 3eXtra Densely Bonded and Double EndcappedEclipse XDB Bonded PhaseZORBAX Eclipse XDB• Four selectivity choices for method development optimization• Good peak shape for basic, acidic and neutral compounds• High performance over a wide pH range – pH 2-9• Particle sizes from 1.8 µm to 7 µm• Long lifetime with extra dense bonding and double endcappingAgilent ZORBAX Eclipse XDB columns – C18, C8, Phenyl and CN – provide four bonded phasechoices for method development optimization. These columns provide good peak shape over awide pH range (2-9) for additional method development flexibility with one family of columns.Eclipse XDB columns can be used for method development at low pH (2-3) and the samecolumn can be used for method development in the mid pH (6-8) region. In the mid pH regionresidual silanols are more active and tailing interactions are more likely. To overcome theseinteractions, Eclipse XDB columns are eXtra Densely Bonded and double endcapped througha proprietary process to cover as many active silanols as possible. The result is superior peakshape of basic compounds from pH 2-9. Eclipse XDB columns are available in 1.8, 3.5, 5and 7 µm particle sizes for high speed, high resolution, analytical and prep scale separations.Column SpecificationsBonded PhasePoreSizeSurfaceAreaTemp.Limits pH Range* EndcappedCarbonLoadZORBAX Eclipse XDB-C18 80Å 180 m 2 /g 60°C 2.0-9.0 Double 10%ZORBAX Eclipse XDB-C8 80Å 180 m 2 /g 60°C 2.0-9.0 Double 7.6%ZORBAX Eclipse 80Å 180 m 2 /g 60°C 2.0-9.0 Double 7.2%XDB-PhenylZORBAX Eclipse XDB-CN 80Å 180 m 2 /g 60°C 2.0-8.0 Double 4.3%Specifications represent typical values only.*Eclipse XDB columns are designed for operation over a wide pH range. At pH 6-9, highest columns stability for all silicabased columns is achieved by operating at temperatures < 40°C and using low buffer concentrations in the range of0.01 – 0.02M.32 Order online at www.agilent.com/chem/store
Columns for Analytical HPLCGood Peak Shape Over a Wide pH Rangewith ZORBAX Eclipse XDBColumn: Eclipse XDB-C8993967-9064.6 x 150 mm, 5 µmMobile Phase: A:pH 3.0 75% 25 mMphosphate bufferpH 3.0: 25% ACNB:pH 7.0 90% 20 mMphosphate pH 7.010% ACNFlow Rate: 1.5 mL/minTemperature: 40°CA12345BSample: A:1. Maleate2. Doxylamine3. Chlorpheniramine4. Triprolidine5. Diphenhydramine2 B:1. Procainamide2. N-acetylprocainamide13. N-propionylprocainamide30 2.5 5.0 7.5Time (min)0 2.5 5.0 7.5Time (min)LCEC004ZORBAX Eclipse XDB columns provide good peak shape over a wide pH range and are anexcellent choice for method development from pH 2-9.Column Stability Testing at pH 7.0Column A: Competitive C8SIL-typeAfter 1826Column VolumesColumn B: Eclipse XDB-C8993967-9064.6 x 150 mm, 5 µmSol-typeAfter 1843Column VolumesMobile Phase: 60% ACN40% 250 mM PhosphateBuffer, pH 7.0Flow Rate: 1.5 mL/minTemperature: 60°CSample:Tricyclic AntidepressantsA1B5 1. Uracil152. Nortriptyline2, 33. Doxepin4. Amitriptyline45. Trimipramine2 340 1 2 3 4 5 6 7 8 9 10 11 12Time (min)0 1 2 3 4 5 6 7 8Time (min)LCEC006Double endcapping, dense bonding and the durable Rx-Sil particles (sol-type) combine to provide long lifetime at pH 7 when compared to single endcapped sil-gel columnsused here. The conditions used for this test – high temperature (60°C) and high salt concentration (250 mM), accelerate the dissolution of silica, causing premature failureof the sil-gel type column.Order online at www.agilent.com/chem/store33
- Page 1 and 2: Agilent HPLCColumn Selection GuideE
- Page 3 and 4: New columns deliver on the promise
- Page 5 and 6: HPLC Column Selection GuidelinesHPL
- Page 7 and 8: HPLC Column Selection GuidelinesQui
- Page 9 and 10: HPLC Column Selection GuidelinesSma
- Page 11 and 12: HPLC Column Selection GuidelinesSil
- Page 13 and 14: HPLC Column Selection GuidelinesQui
- Page 15 and 16: HPLC Column Selection GuidelinesMet
- Page 17 and 18: HPLC Column Selection GuidelinesOpt
- Page 20 and 21: HPLC Column Selection GuidelinesCar
- Page 22 and 23: Columns for Analytical HPLCPoroshel
- Page 24 and 25: Columns for Analytical HPLCEnvironm
- Page 26 and 27: Columns for Analytical HPLCZORBAX E
- Page 28 and 29: Columns for Analytical HPLCRapid An
- Page 30 and 31: Columns for Analytical HPLCZORBAX E
- Page 32 and 33: Columns for Analytical HPLCZORBAX E
- Page 36 and 37: Columns for Analytical HPLCColumn S
- Page 38 and 39: Columns for Analytical HPLCZORBAX E
- Page 40 and 41: Columns for Analytical HPLCZORBAX E
- Page 42 and 43: Columns for Analytical HPLCZORBAX 8
- Page 44 and 45: Columns for Analytical HPLCSB-CN Op
- Page 46 and 47: Columns for Analytical HPLCZORBAX 8
- Page 48 and 49: Columns for Analytical HPLCZORBAX 8
- Page 50 and 51: Columns for Analytical HPLCZORBAX 8
- Page 52 and 53: Columns for Analytical HPLCRx-C8 an
- Page 54 and 55: Columns for Analytical HPLCZORBAX 8
- Page 56 and 57: Columns for Analytical HPLCExtend-C
- Page 58 and 59: Columns for Analytical HPLCR 1O SiP
- Page 60 and 61: Columns for Analytical HPLCZORBAX B
- Page 62 and 63: Columns for Analytical HPLCZORBAX B
- Page 64 and 65: Columns for Analytical HPLCZORBAX N
- Page 66 and 67: Columns for Analytical HPLCNormal P
- Page 68 and 69: Columns for Analytical HPLCZORBAX H
- Page 70 and 71: Columns for Analytical HPLCZORBAX M
- Page 72 and 73: Columns for Analytical HPLCZORBAX M
- Page 74 and 75: Columns for Analytical HPLCCough-Co
- Page 76 and 77: Columns for Special ApplicationsZOR
- Page 78 and 79: Columns for Special ApplicationsSub
- Page 80 and 81: Columns for Special Applications110
- Page 82 and 83: Columns for Special ApplicationsRed
<strong>Column</strong>s for Analytical <strong>HPLC</strong>OOOOOCH 3SiCH 3CH 3Si CH 3CH 3CH 3SiCH 3CH 3Si CH 3CH 3CH 3SiCH 3eXtra Densely Bonded and Double EndcappedEclipse XDB Bonded PhaseZORBAX Eclipse XDB• Four selectivity choices for method development optimization• Good peak shape for basic, acidic and neutral compounds• High performance over a wide pH range – pH 2-9• Particle sizes from 1.8 µm to 7 µm• Long lifetime with extra dense bonding and double endcapping<strong>Agilent</strong> ZORBAX Eclipse XDB columns – C18, C8, Phenyl and CN – provide four bonded phasechoices for method development optimization. These columns provide good peak shape over awide pH range (2-9) for additional method development flexibility with one family of columns.Eclipse XDB columns can be used for method development at low pH (2-3) and the samecolumn can be used for method development in the mid pH (6-8) region. In the mid pH regionresidual silanols are more active and tailing interactions are more likely. To overcome theseinteractions, Eclipse XDB columns are eXtra Densely Bonded and double endcapped througha proprietary process to cover as many active silanols as possible. The result is superior peakshape of basic compounds from pH 2-9. Eclipse XDB columns are available in 1.8, 3.5, 5and 7 µm particle sizes for high speed, high resolution, analytical and prep scale separations.<strong>Column</strong> SpecificationsBonded PhasePoreSizeSurfaceAreaTemp.Limits pH Range* EndcappedCarbonLoadZORBAX Eclipse XDB-C18 80Å 180 m 2 /g 60°C 2.0-9.0 Double 10%ZORBAX Eclipse XDB-C8 80Å 180 m 2 /g 60°C 2.0-9.0 Double 7.6%ZORBAX Eclipse 80Å 180 m 2 /g 60°C 2.0-9.0 Double 7.2%XDB-PhenylZORBAX Eclipse XDB-CN 80Å 180 m 2 /g 60°C 2.0-8.0 Double 4.3%Specifications represent typical values only.*Eclipse XDB columns are designed for operation over a wide pH range. At pH 6-9, highest columns stability for all silicabased columns is achieved by operating at temperatures < 40°C and using low buffer concentrations in the range of0.01 – 0.02M.32 Order online at www.agilent.com/chem/store
Change language