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<strong>Column</strong>s for Analytical <strong>HPLC</strong>Fast and Ultra-Fast Analysisof Basic Compounds on Eclipse Plus<strong>Column</strong> A: Eclipse Plus C18959941-9024.6 x 50 mm, 1.8 µm<strong>Column</strong> B: Eclipse Plus C18959993-9024.6 x 150 mm, 5 µm959993-902Mobile Phase: A: 50% 8 mM K 2 HPO 4 , pH 7B: 50% ACNFlow Rate: 1.0 mL/minTemperature: AmbientDetector: UV 254 nmPublication: 5989-4934ENSample: Berberine, 0.4 mg/mL, 2 µLATf = 1.000 0.5BOO N+ Cl-1. BerberineOCH 3OCH 31 1.5 2 2.5minTf = 1.060 0.5 1 1.5 2 2.5minEP103Order online at www.agilent.com/chem/store27
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