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<strong>Column</strong>s for Analytical <strong>HPLC</strong>Eliminate Tailing and Maximize Resolution with Eclipse Plus<strong>Column</strong>s<strong>Column</strong> A: Eclipse Plus C18, 4.6 x 50 mm1Eclipse Plus C18, 4.6 x 50 mm<strong>Column</strong> B: Competitive C18, 4.6 x 50 mmMobile Phase: 65% ACN: 35% 25 mM phosphate buffer (pH 7.4) Tf= 1.20Superior peak shape and better selectivity with Eclipse Plusmeans more resolution, easier quantitation and better resultsin your separations.Competitive C18, 4.6 x 50 mm11. Nortriptyline2. DipropylthalateTf= 4.86220 0.5 1 1.5 2minEclipse Plus C18 vs. C8 and RRHT vs. RR<strong>Column</strong> A: Eclipse Plus C18959941-9024.6 x 50 mm, 1.8 µm<strong>Column</strong> B: Eclipse Plus C8959943-9064.6 x 50 mm, 3.5 µmMobile Phase: Water: Acetonitrile (30:70)Flow Rate: 2.0 mL/minTemperature: 30°CDetector: UV 230nmSample:Lip balm extract in ACN(melted at 100°C ACN ,cooled and 0.45 µm filtered)AN: 8800Pw : 0.014 min.TF: 1.201. Oxybenzone2. Internal Std.3. OctylmethoxycinnamateN: 11600Pw : 0.047 min.TF: 1.060 1 2 3 4247 barN: 11800Pw : 0.093 min.TF: 1.02minLess retention can save significant time – the C8 is a goodchoice here. The RRHT column is delivering the efficiencyand resolution expected, but the C8 bonded phase may bethe best choice.BN: 5000Pw : 0.019 min.TF: 1.09N: 6600Pw : 0.037 min.TF: 0.99 N: 6300Pw : 0.061 min.TF: 0.9822 min.88 bar0 1 2 3 4minOrder online at www.agilent.com/chem/store25
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