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<strong>Column</strong>s for Analytical <strong>HPLC</strong>ZORBAX Eclipse Plus: Best Peak Shapein the Industry Without Tailing<strong>Column</strong>:Mobile Phase:Flow Rate:Temperature:Detector:Publication:Sample:Eclipse Plus C18959996-9024.6 x 100 mm, 5 µmA: 60% WaterB: 40% Acetonitrile1.0 mL/minAmbientUV 254 nm5989-4934ENPyridine, PhenolEclipse Plus C18Ace 5 C18SunFire C18XBridge C18Discovery HS C18121. Pyridine2. PhenolLuna C18 (2)Gemini C18XTerra MS C180 0.5 1.0 1.5 2.0 2.5 3.0 3.5Time (min)LCEC001Peak Shape and Efficiency are Betterwith ZORBAX Eclipse Plus<strong>Column</strong> A: XBridge C18, 4.6 x 150 mm, 5 µm<strong>Column</strong> B: Eclipse Plus C18959993-9024.6 x 150 mm, 5 µmMobile Phase: A: 0.1% formic acidB: 0.1% formic acid in ACNFlow Rate: 1.0 mL/minGradient: 0.0 min 10% B15 min 30% BTemperature: 40°CDetector: UV 254 nmPublication: 5989-4934ENSample:SulfonamidesA0 2 4 6 8 10 12Time (min)B11N = 14000Tf = 1.252N = 23000Tf = 1.01341. Sulfanilamide2. Sulfadiazine3. Sulfathiazole4. Sulfamerazine5. Sulfmethazine6. Sulfamethoxazole5N = 41000Tf = 1.136N = 60000Tf = 1.00234560 2 4 6 8 10 12Time (min)LCEC00324 Order online at www.agilent.com/chem/store
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