13.07.2015 Views

Agilent HPLC Column Selection Guide

Agilent HPLC Column Selection Guide

Agilent HPLC Column Selection Guide

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<strong>Column</strong>s for Analytical <strong>HPLC</strong>Environmental Phenols on Poroshell 120<strong>Column</strong> A: Poroshell 120 EC-C18695975-9024.6 x 100 mm, 2.7 µm<strong>Column</strong> B: Eclipse Plus C18959964-9024.6 x 100 mm, 1.8 µmGradient:A: Water 0.1% Formic AcidB: Acetonitrile 0.1% formic Acid2 mL/minInitial: 8% B10 min: 30% BDetector:275 nm 2 mm flow cellInjection: 10 µL<strong>Agilent</strong> 1200 SL 40°CNo pulse damperNo mixer 3 µL heaterPoroshell 120 provides sub 2 micron-like efficiency at lower pressure.A123P = 332 Bar1. Hydroquinone 6. o-cresol2. Resorcinol 7. 2-Nitrophenol3. Catechol8. 2,3 Dimethyl phenol4. 4-Nitrophenol 9. 2,5 Dimethyl phenol5. p-cresol10. 1-Naphthol495768100 2 4 6 8 10Time (min)B12P = 510 Bar3495768100 2 4 6 8 10Time (min)EP_Poro12022 Order online at www.agilent.com/chem/store

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