Agilent HPLC Column Selection Guide

Columns for Analytical HPLCPoroshell 120• High efficiency and high resolution• 50% less pressure than sub 2-micron columns• Compatible with 400 bar and 600 bar LCs• Two bonded phases with excellent selectivity and peak shapeAgilent Poroshell 120 columns are a 2.7 µm particle with a 1.7 µm solid core and 0.5 µmporous outer layer. This small particle size provides high efficiency, similar to sub 2-microncolumns, but with 40-50% less pressure. These high efficiency, high resolution columnscan be used on any type of LC. The porous outer layer and solid core limit diffusion distanceand improve separation speed while the narrow particle size distribution improves efficiencyand resolution. The solid core limits diffusion distance and improves separation speed.The columns can support high pressure and multiple columns can be used for the highestresolution and efficiency possible. The same principles are used in the Poroshell 300 columns,ideal for the fast, high resolution separations of biomolecules.Column SpecificationsBonded PhasePore SizeTemp.Limits pH Range EndcappedCarbonLoadSB-C18 120Å 90°C 1.0-8.0 No 8%EC-C18 120Å 60°C 2.0-8.0 Single 10%EC-C8 120Å 60°C 2.0-8.0 Single 5%Specifications represent typical values only.20 Order online at www.agilent.com/chem/store