Agilent HPLC Column Selection Guide
Agilent HPLC Column Selection Guide Agilent HPLC Column Selection Guide
HPLC Column Selection GuidelinesCartridge Column SystemsAgilent offers a variety of popular HPLC packing materials in economical, easy-to-use cartridge configurations.Look for these icons on subsequent pages to help you select the proper guard cartridges and columns.Cartridge Selection GuideIcon Type of Cartridge Features BenefitsAgilent HPLC CartridgeZORBAX Guard Cartridge:Stand alone systemZORBAX Rapid Resolution and RapidResolution HT Cartridge Columns:3.5 µm and 1.8 µm packings,stand alone systemZORBAX Semi-Preparative Guard HPLCHardware Kit:Stand alone systemZORBAX and Agilent Prep PreparativeCartridge Column and Guard HPLC System:Stand alone and integral hardware optionsCan reverse collets in the end fitting to addguard cartridgesCartridges have a unique filter and sieve ateach endHigh efficiency, stand-alone, low deadvolume cartridgePolymeric cartridge designed for leak-tightseals against metal surfacesReusable fittingsFor high throughput LC/MS, LC/MS/MS andcombinatorial separationsPacked with Eclipse XDB for pH use from 2-9Packed with StableBond for low pH useSold individually or as three-packsEasy low-dead-volume assemblyTubing (polyphenylene sulfone) designedfor leak-tight seals against metal surfacesReusable fittingsEasy low-dead-volume assemblyReusable fittingsHardware options for integral andexternal guardsInexpensiveExtends column lifetimePermits rapid column changesCan use 2, 3, 4 and 4.6 mm cartridgesHelps prevent blockageSeals up to 400 barNo gaskets requiredMore solvent-resistant than PEEKAdapt for connections to 1/16 in. LC fittingsFor all analyte typesLow bleedSeals up to 2000 psi (135 bar, 13.5 MPa)No gaskets requiredAdapt for connections to 1/16 in. LC fittingsExtends column lifetimePermits rapid column changesCan use with 21.2 and 30 mm ID columns18 Order online at www.agilent.com/chem/store
Columns for Analytical HPLCAchieve excellent peak shape and resolution –and eliminate "false starts."Good news for analysts who do not have time to "make columns work" for a particularapplication: ZORBAX columns let you choose the right column based on your sampleand mobile phase – eliminating any guesswork.Additionally, Agilent’s ZORBAX silica is manufactured by Agilent – not purchased fromoutside suppliers. And that means we control every step of the manufacturing process,ensuring lot-to-lot consistency, superior performance, and long-term, reliable results.In this section, you will find a diverse range of ZORBAX columns designed for optimumresolution over a wide pH range, including:• Poroshell 120 columns – feature high efficiency and high resolution with 50% less pressurethan sub-2 micron columns.• ZORBAX Eclipse Plus HPLC columns – designed to reliably produce superior peak shapefor basic compounds.• ZORBAX Eclipse PAH HPLC columns – ideal for applications requiring "shape selectivity"or the separation of geometric isomers.• ZORBAX Eclipse XDB HPLC columns – a trusted choice for analytical and regulatory methods.• ZORBAX StableBond HPLC columns – the industry leader for low-pH applications.• ZORBAX Rx HPLC columns – provide excellent stability up to pH 9.• ZORBAX Extend-C18 HPLC columns – feature bidentate bonding that allows you to develophigh-resolution separations at high pH levels.• ZORBAX Bonus-RP HPLC columns – an alkyl-amide column that offers excellent peak shapefor bases, along with an alternative selectivity.• Original ZORBAX HPLC columns – are made with Type A silica and are used in manyestablished separation methods.• ZORBAX HILIC Plus columns – superior peak shape and retention of polar basic compoundswith silica in HILIC mode• ZORBAX Method Development kits – contain three columns for the price of two! Each hasa different bonded phase for optimal selectivity.• ZORBAX Method Validation kits – choose as many columns as you need (or as few) to makemethod validation easier and less expensive.• ZORBAX Ion Exchange columns – available as Strong Anion Exchange (SAX) and StrongCation Exchange (SCX)Order online at www.agilent.com/chem/store19
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<strong>HPLC</strong> <strong>Column</strong> <strong>Selection</strong> <strong>Guide</strong>linesCartridge <strong>Column</strong> Systems<strong>Agilent</strong> offers a variety of popular <strong>HPLC</strong> packing materials in economical, easy-to-use cartridge configurations.Look for these icons on subsequent pages to help you select the proper guard cartridges and columns.Cartridge <strong>Selection</strong> <strong>Guide</strong>Icon Type of Cartridge Features Benefits<strong>Agilent</strong> <strong>HPLC</strong> CartridgeZORBAX Guard Cartridge:Stand alone systemZORBAX Rapid Resolution and RapidResolution HT Cartridge <strong>Column</strong>s:3.5 µm and 1.8 µm packings,stand alone systemZORBAX Semi-Preparative Guard <strong>HPLC</strong>Hardware Kit:Stand alone systemZORBAX and <strong>Agilent</strong> Prep PreparativeCartridge <strong>Column</strong> and Guard <strong>HPLC</strong> System:Stand alone and integral hardware optionsCan reverse collets in the end fitting to addguard cartridgesCartridges have a unique filter and sieve ateach endHigh efficiency, stand-alone, low deadvolume cartridgePolymeric cartridge designed for leak-tightseals against metal surfacesReusable fittingsFor high throughput LC/MS, LC/MS/MS andcombinatorial separationsPacked with Eclipse XDB for pH use from 2-9Packed with StableBond for low pH useSold individually or as three-packsEasy low-dead-volume assemblyTubing (polyphenylene sulfone) designedfor leak-tight seals against metal surfacesReusable fittingsEasy low-dead-volume assemblyReusable fittingsHardware options for integral andexternal guardsInexpensiveExtends column lifetimePermits rapid column changesCan use 2, 3, 4 and 4.6 mm cartridgesHelps prevent blockageSeals up to 400 barNo gaskets requiredMore solvent-resistant than PEEKAdapt for connections to 1/16 in. LC fittingsFor all analyte typesLow bleedSeals up to 2000 psi (135 bar, 13.5 MPa)No gaskets requiredAdapt for connections to 1/16 in. LC fittingsExtends column lifetimePermits rapid column changesCan use with 21.2 and 30 mm ID columns18 Order online at www.agilent.com/chem/store
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