Agilent HPLC Column Selection Guide

Featured Products .......................................................................1HPLC Column Selection Guidelines .............................................3HPLC Column Selection Flow Chart.............................................................................................4Quick Guide to ZORBAX Reversed-Phase Bonded Phases..........................................................5ZORBAX Reversed-Phase HPLC Column Selection Flow Chart ...................................................6Column and Mobile Phase Guidelines: Reversed Phase .............................................................8Quick Guide to USP Designations for HPLC Columns................................................................11Method Development from pH 1-12 .........................................................................................13Cartridge Selection Guide ..........................................................................................................18Columns for Analytical HPLC.....................................................19Poroshell 120 .............................................................................................................................20ZORBAX Eclipse Plus..................................................................................................................23ZORBAX Eclipse PAH .................................................................................................................30ZORBAX Eclipse XDB .................................................................................................................32ZORBAX 80Å StableBond ..........................................................................................................40ZORBAX Rx.................................................................................................................................49ZORBAX 80Å Extend-C18..........................................................................................................52ZORBAX Bonus-RP.....................................................................................................................56ZORBAX Original Reversed Phase Columns ..............................................................................61ZORBAX Normal Phase Columns...............................................................................................62ZORBAX HILIC Plus ....................................................................................................................66ZORBAX Method Development Kits ..........................................................................................68ZORBAX Method Validation Kits ................................................................................................70ZORBAX Ion Exchange Columns – SAX and SCX......................................................................71Columns for Special Applications..............................................73ZORBAX Rapid Resolution High Definition 1.8 µm....................................................................74ZORBAX Rapid Resolution High Throughput 1.8 µm.................................................................77ZORBAX Rapid Resolution 3.5 µm.............................................................................................85ZORBAX Solvent Saver...............................................................................................................90ZORBAX PrepHT.........................................................................................................................94Agilent Prep LC Columns..........................................................................................................100Ultron ES Chiral Columns .........................................................................................................103Columns for Bioanalytical Chromatography ...........................105ZORBAX Strategy for Reversed-PhaseMethod Development of Proteins and Peptides......................................................................106Poroshell 300 ...........................................................................................................................110ZORBAX 300Å StableBond......................................................................................................113ZORBAX 300Å Extend-C18......................................................................................................118ZORBAX Amino Acid Analysis (AAA) Columns and Supplies ..................................................122ZORBAX GF-250 and GF-450 Gel Filtration Columns .............................................................124Agilent Bio-Monolith HPLC Columns .......................................................................................128