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<strong>HPLC</strong> <strong>Column</strong> <strong>Selection</strong> <strong>Guide</strong>linesUSPDesignations USP Packing Materials <strong>Column</strong>sParticleSize (µm)PoreSize (Å)L13L14L20L33Trimethylsilane chemically bonded to porous silica particles,3 to 10 µm in diameterSilica gel 10 µm in diameter with a chemically bonded, strongly basicquaternary ammonium anion-exchange coatingDihydroxypropane groups chemically bonded to porous silica particles,3 to 10 µm in diameterPacking having the capacity to separate proteins by molecular size over a rangeof 4,000 to 400,000 da. It is spherical, silica-based, and processed to providepH stabilityL35A zirconium-stabilizes spherical silica packing with a hydrophilic (diol-type)molecular monolayer bonded phaseL56 Propyl silane chemically bonded to totally porous silica particles, 3 to 10 µmin diameterL57A chiral-recognition protein, ovomucoid, chemically bonded to silica particles,about 5 µm in diameter, with a pore size of 120 angstroms.L60Spherical, porous silica gel, 10 µm or less in diameter, the surface of which hasbeen covalently modified with alkyl amide groups and endcapped.ZORBAX TMS 5 70ZORBAX SAX 4 70ZORBAX GF-250 5 150ZORBAX GF-250 4 150ZORBAX GF-250ZORBAX GF-45046150300ZORBAX SB-C3 3.5, 5 80Ultron ES-OVM 5 120Bonus-RP 1.8, 3.5, 5 8012 Order online at www.agilent.com/chem/store
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