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<strong>Column</strong>s for Bioanalytical Chromatography<strong>Agilent</strong> Bio-Monolith <strong>HPLC</strong> <strong>Column</strong>s• Polymer-based, monolith <strong>HPLC</strong> columns designed for macro bio-molecule separations• Flow rate independent separations; no diffusion, no pores and no void volume maketransport between mobile and stationary phase very rapid• Monolith disc is 5.2 mm x 4.95 mm (100 µL column volume) with continuous channels,eliminating diffusion mass transfer• Extremely fast separations speed up method development time and decrease costs.Locking in method parameters takes significantly less time and buffer<strong>Agilent</strong> Bio-Monolith <strong>HPLC</strong> columns provide high resolution and rapid separations ofantibodies (IgG, IgM), plasmid DNA, viruses, phages and other macro bio-molecules. Theproduct family offers strong cation exchange, strong and weak anion exchange and Protein Aphases. Bio-Monolith <strong>HPLC</strong> columns are compatible with <strong>HPLC</strong> and preparative LC systems,including <strong>Agilent</strong> 1100 and 1200 <strong>HPLC</strong> systems.<strong>Column</strong> SpecificationsDimensions5.2 mm x 4.95 mm<strong>Column</strong> volume 100 µLMaximum pressure150 bar (15 MPa, 2200 psi)Temperature min/max Working: 4°C-40°C (39°F-122°F)Storage: 4°C-30°C (39°F-73°F)Recommended pH Working range: 2-13Cleaning-in-place: 1-14Materials of construction Hardware: Stainless steelPacking: poly (glycidyl methacrylate-co-ethylene dimethacrylate)highly porous monolithColor ring identifierBio-Monolith QA: BlueBio-Monolith DEAE: GreenBio-Monolith SO 3 : RedBio-Monolith Protein A: WhiteShelf life/expiration date Protein A: 12 monthsSO 3 , QA, DEAE: 24-36 months128 Order online at www.agilent.com/chem/store
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