Agilent HPLC Column Selection Guide
Agilent HPLC Column Selection Guide Agilent HPLC Column Selection Guide
Columns for Bioanalytical ChromatographyHigh-Speed Size Exclusion SeparationsColumn: ZORBAX GF-450884973-9029.4 x 250 mm, 6 µmMobile Phase:Flow Rate:Temperature:Detector:Sample:PBS (phosphatebuffered saline), pH 7.43 mL/minAmbient220 nmBSA and BSA DimersHigh-Speed Size Exclusion Separations 2Column: ZORBAX GF-450884973-9029.4 x 250 mm, 6 µmMobile Phase:Flow Rate:Detector:Sample:200 mMNa Phosphate MonobasicpH 7.0/0.1% Azide2 mL/min225 nm10 µg in 50 mMSodium Phosphate pH 7.0A: BSA and BSA Dimers (duplicate injections)21. BSA dimer2. BSA monomer3. Azide2B: Antibody Separation21. IgG Dimer and Aggregate2. Mouse IgG 1K (MOPC 31C)3. Salts: Tris, Na 2 HPO 4 , NaCl13Inj. #1 Inj. #213130 5 10Time (min)0 1 2 3 4 5 6 7 8 9 10Retention Time (min)LCGF002126 Order online at www.agilent.com/chem/store
Columns for Bioanalytical ChromatographyZORBAX GF-250 Shows Extended Column LifetimeStability of GF-250: K* of Standard ProteinsStability of Competitor Column: K* of Standard Proteins3.03.02.52.5K*2.01.51.0BSAOvalbuminLysozymeNaN 3K*2.01.51.0BSAOvalbuminLysozymeNaN 30.50.50.00 1000 2000 3000Column Volumes0.00 1000 2000 3000Column VolumesLCGF004ZORBAX GF-250 and GF-450 Gel Filtration ColumnsHardware DescriptionSize (mm)Particle Size(µm) Part No.GF-250, 150Å 9.4 x 250 4 884973-901GF-250, 150Å 4.6 x 250 4 884973-701GF-450, 300Å 9.4 x 250 6 884973-902Guard Columns (hardware required)GF-250 Diol, Guard Cartridge, 2/pk 9.4 x 15 6 820675-111GF-250 Diol, Guard Cartridge, 4/pk 4.6 x 12.5 6 820950-911GF-450 Diol, Guard Cartridge, 2/pk 9.4 x 15 6 820675-111GF-450 Diol, Guard Cartridge, 4/pk 4.6 x 12.5 6 820950-911Guard Hardware Kit 840140-901Guard Hardware Kit 820888-901PrepHT ColumnsPrepHT GF-250, 150Å 21.2 x 250 6 877974-901PrepHT GF-450, 300Å 21.2 x 250 6 877974-910PrepHT endfittings, 2/pk 820400-901PrepHT GF-250, Guard Cartridge, 2/pk 17 x 7.5 6 820212-911PrepHT GF-450, Guard Cartridge, 2/pk 17 x 7.5 6 820212-911Guard Cartridge Hardware 820444-901Order online at www.agilent.com/chem/store127
- Page 78 and 79: Columns for Special ApplicationsSub
- Page 80 and 81: Columns for Special Applications110
- Page 82 and 83: Columns for Special ApplicationsRed
- Page 84 and 85: Columns for Special ApplicationsRap
- Page 86 and 87: Columns for Special ApplicationsRap
- Page 88 and 89: Columns for Special ApplicationsRun
- Page 90 and 91: Columns for Special ApplicationsZOR
- Page 92 and 93: Columns for Special ApplicationsZOR
- Page 94 and 95: Columns for Special ApplicationsZOR
- Page 96 and 97: Columns for Special ApplicationsZOR
- Page 98 and 99: Columns for Special ApplicationsSca
- Page 100 and 101: Columns for Special ApplicationsZOR
- Page 102 and 103: Columns for Special ApplicationsAgi
- Page 104 and 105: Columns for Special ApplicationsAgi
- Page 106 and 107: Columns for Special ApplicationsSep
- Page 108 and 109: Columns for Bioanalytical Chromatog
- Page 110 and 111: Columns for Bioanalytical Chromatog
- Page 112 and 113: Columns for Bioanalytical Chromatog
- Page 114 and 115: Columns for Bioanalytical Chromatog
- Page 116 and 117: Columns for Bioanalytical Chromatog
- Page 118 and 119: Columns for Bioanalytical Chromatog
- Page 120 and 121: Columns for Bioanalytical Chromatog
- Page 122 and 123: Columns for Bioanalytical Chromatog
- Page 124 and 125: Columns for Bioanalytical Chromatog
- Page 126 and 127: Columns for Bioanalytical Chromatog
- Page 130 and 131: Columns for Bioanalytical Chromatog
- Page 132 and 133: Columns for Bioanalytical Chromatog
- Page 134 and 135: Technical Support at work for youHa
- Page 136: How to contact AgilentFor the lates
<strong>Column</strong>s for Bioanalytical ChromatographyHigh-Speed Size Exclusion Separations<strong>Column</strong>: ZORBAX GF-450884973-9029.4 x 250 mm, 6 µmMobile Phase:Flow Rate:Temperature:Detector:Sample:PBS (phosphatebuffered saline), pH 7.43 mL/minAmbient220 nmBSA and BSA DimersHigh-Speed Size Exclusion Separations 2<strong>Column</strong>: ZORBAX GF-450884973-9029.4 x 250 mm, 6 µmMobile Phase:Flow Rate:Detector:Sample:200 mMNa Phosphate MonobasicpH 7.0/0.1% Azide2 mL/min225 nm10 µg in 50 mMSodium Phosphate pH 7.0A: BSA and BSA Dimers (duplicate injections)21. BSA dimer2. BSA monomer3. Azide2B: Antibody Separation21. IgG Dimer and Aggregate2. Mouse IgG 1K (MOPC 31C)3. Salts: Tris, Na 2 HPO 4 , NaCl13Inj. #1 Inj. #213130 5 10Time (min)0 1 2 3 4 5 6 7 8 9 10Retention Time (min)LCGF002126 Order online at www.agilent.com/chem/store
Change language