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<strong>Column</strong>s for Bioanalytical ChromatographySeparation of Protein Standardson the ZORBAX GF-250 SEC <strong>Column</strong>5<strong>Column</strong>: ZORBAX GF-250884973-9019.4 x 250 mm, 4 µmMobile Phase: 200 mM Sodium Phosphate, pH 7.0Flow Rate: 2 mL/minTemperature: AmbientDetector: 254 nmThe protein standards separated here are a commonly selected set ofstandards. The ZORBAX GF-250 column shows excellent resolution forthis sample. Additional resolution of the thyroglobulin can be obtained byadding the GF-450 column in series.Sample:BioRad Gel Filtration Standardsfor Size Exclusion1. Thyroglobulin 670,000 Da2. Bovine Gamma Globulin 158,000 Da3. Chicken Ovalbumin 44,000 Da4. Equine Myoglobin 17,000 Da5. Vitamin B-12 1,350 Da12340 1 2 3 4 5 6 7 8Time (min)LCGF001Order online at www.agilent.com/chem/store125
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