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<strong>Column</strong>s for Bioanalytical ChromatographyAmino Acid StandardsEach amino acid standard containsthe following amino acids:• Glycine• L-cystine• L-histidine• L-tyrosine• L-leucine• L-methionine• L-serine• L-alanine• L-phenylalanine• L-glutamic acid• L-proline• L-isoleucine• L-arginine• L-threonine• L-valine• L-lysine• L-aspartic acidZORBAX Amino Acid Analysis (AAA)<strong>Column</strong>s and SuppliesZORBAX Eclipse Amino Acid Analysis (AAA) <strong>Column</strong>s• High resolution and rapid analysis of 24 amino acids• Use tested for amino acid analysis• Uses well known OPA and FMOC precolumn derivatization chemistry• Easily automated using a detailed online, derivatization protocol available for use with<strong>Agilent</strong> 1100/1200 AutosamplerThe <strong>Agilent</strong> ZORBAX Eclipse AAA high efficiency column rapidly separates amino acidsfollowing an updated and improved protocol. Total analysis from injection to injection can beachieved in as little as 14 min. (9 min. analysis time) on shorter, 7.5 cm length columns and24 min. (18 min. analysis time) on the 15 cm column length. Exceptional sensitivity (5-50pmol with DAD, FLD) and reliability are achieved using both OPA and FMOC derivatizationchemistries in one fully automated procedure using the <strong>Agilent</strong> 1100/1200 <strong>HPLC</strong> instrument.ZORBAX Eclipse Amino Acid Analysis (AAA) <strong>Column</strong>sParticle SizeHardware Description Size (mm) (µm) Part No.Analytical routine sensitivity 4.6 x 150 5 993400-902Analytical routine sensitivity, 4.6 x 150 3.5 963400-902high-resolution using FLDAnalytical routine sensitivity, 4.6 x 75 3.5 966400-902high-throughputSolvent Saver high sensitivity, 3.0 x 150 3.5 961400-302high resolutionGuard Catridges, 4/pk 4.6 x 12.5 5 820950-931Guard Hardware Kit 820888-901122 Order online at www.agilent.com/chem/store
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